B9139
Biotin-dextran
mol wt 10,000, Lysine-fixable
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About This Item
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conjugate
biotin conjugate
Quality Level
form
powder
mol wt
10,000
extent of labeling
~2 mol per mol
color
white
solubility
H2O: soluble 50 mg/mL, clear, colorless to faintly yellow
storage temp.
2-8°C
Application
Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction.[1] Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts.[2] Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).[3]
Other Notes
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
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This Item | |||
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| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| solubility H2O: soluble 50 mg/mL, clear, colorless to faintly yellow | solubility water: 50 mg/mL, clear to slightly hazy, colorless to faintly yellow | solubility H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange | solubility H2O: soluble 100 mg/mL, clear, colorless to faintly yellow |
| form powder | form powder | form powder | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. room temp |
| color white | color - | color yellow to orange | color - |
| conjugate biotin conjugate | conjugate - | conjugate FITC conjugate | conjugate - |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Yang-Can Duan et al.
Pflugers Archiv : European journal of physiology, 459(1), 1-9 (2009-07-25)
This study is to determine whether the nucleus of solitary tract (NTS) is an important component of the central neurocircuitry of the cardiac sympathetic afferent reflex (CSAR) and whether the gamma-aminobutyric acid (GABA) in the NTS modulates the CSAR. Renal
Shih-Yen Tsai et al.
Stroke, 42(1), 186-190 (2010-11-23)
we have shown that anti-Nogo-A immunotherapy to neutralize the neurite growth inhibitory protein Nogo-A results in functional improvement and enhanced plasticity after ischemic stroke in the adult rat. The present study investigated whether functional improvement and neuronal plasticity can be
Jia Liu et al.
Journal of the neurological sciences, 325(1-2), 127-136 (2013-01-16)
The stem cell-based experimental therapies are partially successful for the recovery of spinal cord injury (SCI). Recently, acellular spinal cord (ASC) scaffolds which mimic native extracellular matrix (ECM) have been successfully prepared. This study aimed at investigating whether the spinal
Global Trade Item Number
| SKU | GTIN |
|---|---|
| B9139-25MG | 04061833031865 |
| B9139-10MG | 04061833031858 |