This product has not been tested on microalgae.
APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Synonym(s):
Red fluorescent Annexin V apoptosis assay
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About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
Pricing and availability is not currently available.
usage
sufficient for 200 tests
Quality Level
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Legal Information
Cy3 is a trademark of Cytiva
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This Item | |||
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) protein quantification: suitable | technique(s) - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit |
| usage sufficient for 200 tests | usage (20 tests) | usage 1 kit sufficient for 2,000 tests | usage 1 kit sufficient for 1,000 tests |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| detection method fluorometric | detection method fluorometric | detection method colorimetric, fluorometric | detection method colorimetric |
Kit Components Only
Product No.
Description
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| APOAC-1KT | 04061833418819 |
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can we use this kit used for microalgae cells
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