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4-Aminophenyl β-D-galactopyranoside

Name: 4-Aminophenyl β-<SC>D</SC>-galactopyranoside
Brand: SIGMA

≥98% (TLC), β-galactosidase substrate

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About This Item

Empirical Formula (Hill Notation):

C₁₂H₁₇NO₆

CAS Number:

5094-33-7

Molecular Weight:

271.27

NACRES:

NA.25

PubChem Substance ID:

24891470

UNSPSC Code:

12352201

MDL number:

MFCD00067362

Pricing and availability is not currently available.

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Properties

Quality Level

200

assay

≥98% (TLC)

form

powder

optical activity

[α]/D -42.00 to -34.00°, c = 9.00-11.00 mg/mL in water

technique(s)

thin layer chromatography (TLC): suitable

color

white to yellow cast

solubility

water: 49.00-51.00 mg/mL, clear, colorless to yellow

storage temp.

−20°C

SMILES string

Nc1ccc(OC2OC(CO)C(O)C(O)C2O)cc1

InChI

1S/C12H17NO6/c13-6-1-3-7(4-2-6)18-12-11(17)10(16)9(15)8(5-14)19-12/h1-4,8-12,14-17H,5,13H2

InChI key

MIAKOEWBCMPCQR-UHFFFAOYSA-N

Description

Application

4-Aminophenyl β-D-galactopyranoside (PAPG, 4-APG) is used as a substrate for the development of β-galactosidase(s) (GUS)-dependent assay systems via the formation of an electroactive product. It may be used to create affinity matricies for the capture or purification of β-galactosidase(s).[1][2]

Other Notes

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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This Item	A1394	M9271	G0259
Sigma-Aldrich A9545 4-Aminophenyl β-D-galactopyranoside	Sigma-Aldrich A1394 4-Aminophenyl α-D-mannopyranoside	Sigma-Aldrich M9271 α-D-Mannopyranosylphenyl isothiocyanate	Sigma-Aldrich G0259 D-Glucosaminic acid
technique(s) thin layer chromatography (TLC): suitable	technique(s) thin layer chromatography (TLC): suitable	technique(s) thin layer chromatography (TLC): suitable	technique(s) thin layer chromatography (TLC): suitable
assay ≥98% (TLC)	assay ≥98% (TLC)	assay >98% (TLC)	assay ≥98% (TLC)
solubility water: 49.00-51.00 mg/mL, clear, colorless to yellow	solubility H₂O: soluble	solubility 50 mg/mL, clear to very slightly hazy, colorless to light yellow (ACETONE:DIMETHYL SULFOXIDE (7:3))	solubility 0.5 M HCl: 50 mg/mL, clear, colorless to faintly yellow
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
form powder	form powder	form powder	form powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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The adjuvant effect of liposomes in eliciting anti-galactosyl antibodies.

D P Sarkar et al.

Immunological communications, 11(3), 175-188 (1982-01-01)

Negatively-charged, multilamellar liposomes, covalently coupled with p-aminophenyl-beta-D-galactopyranoside, elicited the rabbits a nearly equivalent anti-galactosyl immune response in both the presence and the absence of complete Freund's-adjuvant (CFA). The antibody response obtained using liposomes both as the carrier and adjuvant was

Fast electrochemical detection of anti-HIV antibodies: coupling allosteric enzymes and disk microelectrode arrays.

Olivier Laczka et al.

Analytica chimica acta, 641(1-2), 1-6 (2009-04-28)

Here a novel electrochemical method for the rapid detection of anti-HIV antibodies in serum is presented. The novelty lies in the combination of allosteric enzymes and coulometry to yield a fast, simple and reliable HIV diagnostic method. We have used

[Chemical attachment of p-aminophenylgalactoside to acid alpha-glucosidase from human liver].

D M Belen'kiĭ et al.

Biokhimiia (Moscow, Russia), 47(7), 1141-1146 (1982-07-01)

Chemical modification of the COOH-groups of acid alpha-glucosidase from human liver by 1-ethyl-3 (3'-dimethylaminopropyl) carbodiimide. HCl in the presence of rho-aminophenyl-beta-D-galactopyranoside was carried out. The presence of covalently bound galactose derivative in the enzyme was followed by changes in the

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Global Trade Item Number

SKU	GTIN
A9545-1G	04061833403365
A9545-100MG	04061832387185

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