A7189
L-Alanine Dehydrogenase from Bacillus subtilis
ammonium sulfate suspension, ≥20 units/mg protein (Lowry)
Synonym(s):
L-Alanine: NAD+ oxidoreductase (deaminating)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
Specific activity:
≥20 units/mg protein (Lowry)
Biological source:
Bacillus subtilis
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biological source
Bacillus subtilis
Quality Segment
form
ammonium sulfate suspension
specific activity
≥20 units/mg protein (Lowry)
storage temp.
2-8°C
General description
L-Alanine Dehydrogenase has a N-terminal substrate-binding domain and a C-terminal NAD-binding domain.
Application
L-Alanine Dehydrogenase from Bacillus subtilis has been used in the carbon nanotube columns for H2-driven biocatalysis hydrogenation studies.
L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection [1].
Biochem/physiol Actions
L-Alanine Dehydrogenase is essential for sporulation in Bacillus subtilis.
L-Alanine dehydrogenase is a stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate and NADH are released in that specific order.[1] Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.[2]
Physical form
Suspension in 2.4 M (NH4)2SO4 solution, pH 7.0
Other Notes
One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.
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This Item | |||
|---|---|---|---|
| specific activity ≥20 units/mg protein (Lowry) | specific activity ~30 units/mg protein (Lowry) | specific activity ≥250 units/mg protein (biuret) | specific activity ≥60 units/mg protein |
| biological source Bacillus subtilis | biological source Bacillus subtilis | biological source - | biological source - |
| form ammonium sulfate suspension | form buffered aqueous glycerol solution | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A7189-250UN | 04061838168511 |
| A7189-500UN | 04061838168528 |