A1520

α-Amylase Inhibitor

≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase), ≥1000 inhibitor U/mg protein (using human salivary α-amylase), lyophilized powder

• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00130475

Properties

• Product Name: α-Amylase Inhibitor from Triticum aestivum (wheat seed), Type I, lyophilized powder
• biological source: Triticum estivum
• Quality Segment: 200
• type: Type I
• form: lyophilized powder
• specific activity: ≥1000 inhibitor U/mg protein (using human salivary α-amylase), ≥200 inhibitor U/mg protein (using porcine pancreatic α-amylase)
• composition: Protein, 35-65% biuret
• storage temp.: −20°C

Description

General description

α-Amylases are endoglycosidases, that are classified into glycosyl hydrolase family 13 (1-3).

Application

α-Amylase Inhibitor from Triticum aestivum (wheat seed) has been used to determine its effects on salivary gland (SG) amylase activity. It has also been used to inhibit the activity from the extract from Bemisia tabaci.

Biochem/physiol Actions

Competitive inhibitor of human salivary α-amylase. K_I = 2.9 nM, compared to a K_M of 5.9 mM (calculated per mole of α-1,4-linked maltose residues).

α-Amylases can hydrolyze α-(1,4)-D-glycosidic linkages. It participates in the breakdown of starch and glycogen. Inhibiting this enzyme can be used as a strategy to treat dental caries, diabetes, obesity and periodontal diseases.

Physical form

Lyophilized powder containing buffer salts as sodium phosphate.

Other Notes

One unit will reduce the activity of two units of α-amylase (A0521) by 50% after pre-incubation at 25 °C.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)