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ABTS Enhancer

Name: ABTS Enhancer
Brand: SIGMA

liquid, for peroxidase detection

Synonym(s):

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Enhancer

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.83

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Properties

Product Name

ABTS Enhancer, for peroxidase detection

Quality Segment

100

form

liquid

storage temp.

2-8°C

Description

Application

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) has been used for the determination of laccase activity in Trametes versicolor.[1] It may be suitable for use in enzyme-linked immunosorbent assay (ELISA) for detecting signal strength emitted by cells.[2]

Biochem/physiol Actions

2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) is a non-phenolic substrate.[3] It might exhibit antioxidant property. The detection of the horseradish peroxidase substrate ABTS can be improved by the addition of a chemical enhancer. The ABTS enhancer increases the absorbance of the oxidized product 50-150%. This increase is used to improve assay sensitivity or conserve assay components.

Physical form

Supplied as an 11X concentrate

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1 of 1

This Item	11557	A9941	A1888
Sigma-Aldrich A1227 ABTS Enhancer	Sigma-Aldrich 11557 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	Sigma-Aldrich A9941 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt	Sigma-Aldrich A1888 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 200
form liquid	form powder or crystals	form tablet	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. room temp	storage temp. 2-8°C

pictograms

GHS08

signalword

Danger

hcodes

H350,H412

pcodes

P201 - P273 - P308 + P313

Hazard Classifications

Aquatic Chronic 3 - Carc. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
A1227-10ML	04061838251251

Questions

1–3 of 3 Questions

Anonymous

4 years ago

What is the Department of Transportation shipping information for this product?

1 answer
1. Technical Support
  
  4 years ago
  
  Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
  
  Helpful?
Anonymous

4 years ago

How can Product A1227, ABTS Enhancer, be used?

1 answer
1. Technical Support
  
  4 years ago
  
  This product is for microwell applications such as ELISA. A soluble reaction product is produced, so this is not for applications that require a precipitation reaction such as blotting or histochemical applications.
  
  Helpful?
Anonymous

4 years ago

Why should Product A1227, ABTS Enhancer, be used?

1 answer
1. Technical Support
  
  4 years ago
  
  This enhancer may be used to increase sensitivity or to conserve assay components.
  
  Helpful?

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