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38361

Abberior® STAR 440SXP, maleimide

for long Stokes STED and 2-color STED application

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NACRES:
NA.32
UNSPSC Code:
12352111
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Quality Segment

assay

≥80.0% (degree of coupling)

mol wt

Mw 622.6 g/mol

solubility

DMF: 1 mg/mL, clear

fluorescence

λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4

storage temp.

−20°C

General description

Absorption Maximum, λmax: 433 nm (MeOH)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)

Application

Abberior® STAR 440SXP has been conjugated with secondary anti-mouse antibody for dual colour STED (stimulated emission depletion) microscopy.[1] Again, it has been labelled with secondary antibody for STED microscopy in primary hippocampal cells prepared from E18 Sprague Dawley embryos.[2]
Designed and tested for fluorescent super-resolution microscopy

Legal Information

abberior is a registered trademark of Abberior GmbH

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1 of 1

This Item
682210300430558
fluorescence

λex 437 nm; λem 515 nm±5 nm in PBS, pH 7.4

fluorescence

λex 440 nm; λem 511 nm±10 nm in PBS, pH 7.4

fluorescence

λex 512 nm; λem 530 nm±5 nm in PBS, pH 7.4

fluorescence

λex 635 nm; λem 655 nm±10 nm in PBS, pH 7.4

solubility

DMF: 1 mg/mL, clear

solubility

DMF: 1 mg/mL, clear

solubility

DMF: 0.25 mg/mL, clear

solubility

DMF: 0.25 mg/mL, clear

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

assay

≥80.0% (degree of coupling)

assay

≥80.0% (degree of coupling)

assay

-

assay

-

mol wt

Mw 622.6 g/mol

mol wt

Mw 597.5 g/mol

mol wt

Mw 861.7 g/mol

mol wt

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Linda Westin et al.
BMC neuroscience, 15, 45-45 (2014-03-29)
Norbin is a neuron-specific, cytosolic protein that interacts with the metabotropic glutamate receptor 5 (mGluR5) and has a profound impact on mGluR5 signaling. Yet, little is known about its synaptic distribution. Here we have analyzed the spatial relationship between Norbin
I-Hsuan Wang et al.
Cell host & microbe, 14(4), 468-480 (2013-10-22)
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported



Global Trade Item Number

SKUGTIN
38361-1MG04061823366212

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