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CHAPS 100 mM solution

Synonym(s):

CHAPS solution

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About This Item

Empirical Formula (Hill Notation):
C32H58N2O7S
CAS Number:
Molecular Weight:
614.88
NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
12161902
MDL number:
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description

zwitterionic

Quality Segment

form

liquid

mol wt

614.88 g/mol

concentration

100 mM in H2O

technique(s)

protein quantification: suitable

CMC

8 mM

transition temp

cloud point >100 °C

density

1.01 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-RFCNGIAKSA-N

General description

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) is a biocompatible surfactant. It is a derivative of cholic acid comprising a hydrophobic cholesterol-like skeleton possessing three hydroxyl groups and a zwitterionic aliphatic tail.[1]

Application

CHAPS 100 mM solution has been used as a component of lysis buffer for the extraction of proteins from goat endometrial epithelial cells (EECs)[2] and liver tissue.[3]

Biochem/physiol Actions

3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) exhibits the properties of both sulfobetaine-type surfactants and bile salts. It is involved in the protein extraction process for proteomic analysis and reconstitution of membrane proteins into lipid vesicles (proteoliposomes). CHAPS is broadly used in biochemistry for membrane protein and receptor isolation and purification. It is conveniently used in pharmaceuticals and medicines due to its biocompatibility, noncharged character, nondenaturing, and disaggregating properties.[1]

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This Item
CHAPS-RO3050-OP1.11662
technique(s)

protein quantification: suitable

technique(s)

dialysis: suitable

technique(s)

-

technique(s)

-

form

liquid

form

crystals

form

crystalline powder

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

10-30°C

storage temp.

2-8°C

CMC

8 mM

CMC

4 mM, 6 mM (20-25°C)

CMC

6 mM (20-25°C)

CMC

6 mM (20-25°C)

mol wt

614.88 g/mol

mol wt

micellar avg mol wt 6150, 614.88 g/mol (anhydrous basis)

mol wt

micellar avg mol wt 6150

mol wt

micellar avg mol wt 6150

density

1.01 g/mL at 20 °C

density

-

density

-

density

-


Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Youichi Shinozaki et al.
PLoS biology, 7(5), e1000103-e1000103 (2009-05-08)
The ATP-gated P2X(4) receptor is a cation channel, which is important in various pathophysiological events. The architecture of the P2X(4) receptor in the activated state and how to change its structure in response to ATP binding are not fully understood.
Justyna Kolakowska et al.
Reproduction, fertility, and development, 29(11), 2255-2268 (2017-04-19)
Reproductive processes in domestic pigs have been studied extensively. Pigs are one of the main sources of meat for human consumption and are an established model for investigations into mammalian, including human, reproductive physiology. Studies of the uterus during early
Yao Zhu et al.
Foods (Basel, Switzerland), 8(2) (2019-02-16)
RNAlater® is regarded as a potential preservation method for proteins, while its effect on bovine muscle proteins has rarely been evaluated. Bovine muscle protein samples (n = 12) collected from three tender (Warner⁻Bratzler shear force: 30.02⁻31.74 N) and three tough



Questions

  1. What is the stability time of this product at 2-8°C?

    1 answer
    1. This product is not assigned an expiration or retest date. Products that are robust in nature and extremely stable, in the original unopened container, are not assigned an expiration date or retest schedule. These products will have no dating reported on either the label or the Certificate of Analysis.
      Products that are not assigned an expiration or retest date are covered by a warranty period of 1 year from the date of product shipment.
      Please see the link below to review the complete Product Dating Information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/190/408/product-dating-information-ms.pdf

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