This enzyme is a recombinant protein produced in e. coli. The biological source and sequence of the protein is considered proprietary and may not be shared.
04529
Nitrilase
recombinant, expressed in E. coli, ≥2.0 U/mg
Synonym(s):
Nitrile aminohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥2.0 U/mg
Recombinant:
expressed in E. coli
Pricing and availability is not currently available.
recombinant
expressed in E. coli
form
crystals, powder
specific activity
≥2.0 U/mg
mol wt
41 kDa
storage temp.
2-8°C
General description
Application
Nitrilase may be used for immobilization on acryloyl crosslinked cellulose dialdehyde (ACCD) for catalytic activity studies.[3]
Biochem/physiol Actions
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
1 U corresponds to the amount of enzyme which liberates 1 μmol ammonia per minute at pH 7.2 and 25°C with the conversion of acrylonitrile to acrylic acid
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥2.0 U/mg | specific activity ≥50 units/mg protein | specific activity ≥20,000 U/g | specific activity ≥130 units/mg protein |
| form crystals, powder | form lyophilized powder | form lyophilized | form buffered aqueous glycerol solution |
| recombinant expressed in E. coli | recombinant - | recombinant - | recombinant - |
| mol wt 41 kDa | mol wt 110 kDa | mol wt - | mol wt 32.5-33 kDa |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Zhi-Qiang Liu et al.
PloS one, 8(6), e67197-e67197 (2013-07-05)
In this study, several nitrilase genes from phylogenetically distinct organisms were expressed and purified in E. coli in order to study their ability to mediate the biotransformation of nitriles. We identified three nitrilases: Acidovorax facilis nitrilase (AcN); Alcaligenes fecalis nitrilase
Chunsheng Yang et al.
Applied biochemistry and biotechnology, 165(7-8), 1556-1567 (2011-09-23)
The characteristics of the new nitrilase-producing strain Rhodobacter sphaeroides LHS-305 were investigated. By investigating several parameters influencing nitrilase production, the specific cell activity was ultimately increased from 24.5 to 75.0 μmol g(-1) min(-1), and hereinto, the choice of inducer proved
Pablo Soriano-Maldonado et al.
Applied and environmental microbiology, 77(16), 5761-5769 (2011-06-28)
Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 238430-10G | 04061838347695 |
| 238430-50G | 04061838786890 |
| 04529-50MG | 04061833227930 |
| 04529-10MG | 04061833227923 |
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what is the bacteria or organism whose genome sequence have been expressed in E.Coli for nitrilase procurement? I need the name of the organism for research purpose
1 answer-
Helpful?
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Can we get the source of the bacteria or organism whose genome sequence is being used for recombinant expressing in E.Coli for procuring nitrilase. Used for research purspose by a student
1 answer-
Unfortunately, the source and sequence is considered proprietary.
Helpful?
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Is the nitrilase been synthesized through DNA recombinant technology or is there any other source organism from which the nitrilase has been procured?
1 answer-
Yes, this product is a recombinant protein expressed in E. coli.
Helpful?
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