Unfortunately, sequencing has not been performed on this material.
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-732-3
MDL number:
Specific activity:
>0.5 U/mg
Pricing and availability is not currently available.
General description
Acylase I belongs to the aminoacylase family of enzymes.
Application
Acylase I from Aspergillus melleus has been used to catalyze the Mannich reaction.
Biochem/physiol Actions
Acylase I plays an important role in the amino acid metabolism of organisms. It also plays a role in the acylation of primary and secondary alcohols. Acylase I is involved in alcoholysis.
Analysis Note
Enzyme activity: the optimum temperature is 40-45°C, the optimum pH is 8.0 (stable form pH 6-10). The enzyme is activated by CoCl2 in the range of 10-4 to 10-3 M.
Other Notes
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 37°C
Resolution of acetyl amino acids
1 of 1
This Item | |||
|---|---|---|---|
| specific activity >0.5 U/mg | specific activity ≥3 units/mg solid | specific activity ~30 U/mg | specific activity ≥8.0 units/mg solid |
| form powder | form solid | form powder | form solid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| color brown | color - | color - | color - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Enzyme-catalyzed asymmetric Mannich reaction using acylase from Aspergillus melleus
Guan Z, et al.
Journal of Molecular Catalysis. B, Enzymatic, 111, 16-20 (2015)
Preparation of the enantiomers of 1-phenylethan-1, 2-diol. Regio-and enantioselectivity of acylase I and Candida antarctica lipases A and B
Virsu P, et al.
Tetrahedron, 12(17), 2447-2455 (2001)
K. Uchida et al.
Journal of Labelled Compounds & Radiopharmaceuticals, 29, 867-867 (1991)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 132470-25G | 04061838729002 |
| 132470-100G | 04061838345820 |
| 01818-25G | 04061838160416 |
| 01818-5G | 04061838610140 |
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What is the amino acid or DNA sequence of the Aspergillus melleus acylase I?
1 answer-
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