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01818

Acylase I from Aspergillus melleus

powder, brown, >0.5 U/mg

Synonym(s):

Aminoacylase, Acylase Amano

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About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-732-3
MDL number:
EC Number:
Specific activity:
>0.5 U/mg
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form

powder

Quality Level

specific activity

>0.5 U/mg

color

brown

storage temp.

2-8°C

General description

Acylase I belongs to the aminoacylase family of enzymes.

Application

Acylase I from Aspergillus melleus has been used to catalyze the Mannich reaction.

Biochem/physiol Actions

Acylase I plays an important role in the amino acid metabolism of organisms. It also plays a role in the acylation of primary and secondary alcohols. Acylase I is involved in alcoholysis.

Analysis Note

Enzyme activity: the optimum temperature is 40-45°C, the optimum pH is 8.0 (stable form pH 6-10). The enzyme is activated by CoCl2 in the range of 10-4 to 10-3 M.

Other Notes

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 37°C
Resolution of acetyl amino acids

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This Item
P403210065G5160
specific activity

>0.5 U/mg

specific activity

≥3 units/mg solid

specific activity

~30 U/mg

specific activity

≥8.0 units/mg solid

form

powder

form

solid

form

powder

form

solid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

color

brown

color

-

color

-

color

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Enzyme-catalyzed asymmetric Mannich reaction using acylase from Aspergillus melleus
Guan Z, et al.
Journal of Molecular Catalysis. B, Enzymatic, 111, 16-20 (2015)
Preparation of the enantiomers of 1-phenylethan-1, 2-diol. Regio-and enantioselectivity of acylase I and Candida antarctica lipases A and B
Virsu P, et al.
Tetrahedron, 12(17), 2447-2455 (2001)
K. Uchida et al.
Journal of Labelled Compounds & Radiopharmaceuticals, 29, 867-867 (1991)



Global Trade Item Number

SKUGTIN
132470-25G04061838729002
132470-100G04061838345820
01818-25G04061838160416
01818-5G04061838610140

Questions

  1. What is the amino acid or DNA sequence of the Aspergillus melleus acylase I?

    1 answer
    1. Unfortunately, sequencing has not been performed on this material.

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