SCC421

VCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line

Human

• Synonym(s): Enzalutamide-resistant prostate cancer cells
• UNSPSC Code: 41106514
• NACRES: NA.81
• Biological source: human
• Growth mode: N/A
• Manufacturer/tradename: Millipore

Properties

• Product Name: VCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line,
• biological source: human
• packaging: vial of >1X10⁶ cells
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: ≤ − 140°C

Description

Application

Prostate cancer (PCA) is the most frequently diagnosed cancer among men in the USA, with over 150 000 new cases expected annually. Approximately 6% become metastatic, with a 5-yr survival rate of only 29%. Metastatic lesions form most frequently in bone. Since the 1940s, the standard treatment for advanced PCA has been surgical or chemical castration targeting the androgen receptor (AR) signaling axis. Most treated patients, however, develop resistance and become androgen independent, which contributes to disease progression and poor prognosis, delineating a critical need to identify novel targetable pathways mediating resistance (1). The scientific investigation of PCA tumorigenesis and experimental therapies has been limited to a few of cell- and animal-based model systems.

Source:
Xenografted into SCID mice before propagated in cell culture, the parental PCA cell line VCaP derives from a vertebral metastatic lesion of a hormone refractory prostate cancer patient. Successfully cultured for over 50 passages in vitro, VCaP is considered an immortalized cell line (2). VCaP-EnzR is an enzalutamide resistant (EnzR) derivative of VCaP, developed by prolonged in vitro culture with enzalutamide, a second-generation AR antagonist efficacious in the treatment of castration resistant prostate cancer. VcaP-EnzR is a valuable research tool for studying castration resistant prostate cancer (3).

Biosafety Level 2
VCaP-EnzR cells contain mouse xenotropic retrovirus Bxv-1 and should be handled under biosafety level 2. The parental VCaP prostate cancer cell line is known to produce the mouse xenotropic rerovirus Bxv-1, which was likely acquired by the cells during their xenotransplantation in mice.

References:
1. Kelly SP et al. (2018) Past, current and future incidence rates and burden of metastatic prostate cancer in the United States. Eur Urol Focus 4(1):121–127.
2. Korenchuk S et al. (2001) VcaP, a cell-based model system of human prostate cancer. In Vivo 15(2):163-168.
3. Kregel S et al. (2016) Acquired resistance to the second-generation androgen receptor antagonist enzalutamide in castration-resistant prostate cancer. Oncotarget 7(18):26259-26274.

Features and Benefits

VCaP-EnzR Human Enzalutamide Resistant Prostate Cell Line is a valuable research model for studying castration resistant prostate cancer

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable