NS170

Multi Species Phosphorylated Neurofilament ELISA Kit

measures and quantifies pNF-H levels in serum or plasma

• Synonym(s): 200 kDa neurofilament protein, Phosphorylated Neurofilament heavy polypeptide, pNF-H
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84
• Species reactivity: pig, feline, rat, human, bovine, mouse
• Shipped in: dry ice

Properties

• Product Name: Phosphorylated Neurofilament (pNF-H) Sandwich ELISA Kit, The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.
• Quality Level: 200
• species reactivity: pig, feline, rat, human, bovine, mouse
• manufacturer/tradename: Chemicon^®
• assay range: sensitivity: 0.0293-15 ng/mL; (Range of Detection), sensitivity: 0.0585 ng/mL
• technique(s): ELISA: suitable
• NCBI accession no.: NM_021076.2
• UniProt accession no.: P12036
• application(s): research use
• detection method: colorimetric
• shipped in: dry ice

Description

Application

Research Category
Neuroscience

The Phosphorylated Neurofilament H (pNF-H) kit is a sandwich enzyme immunoassay (EIA), which measures pNF-H.

Used to detect/quantify: Phosphorylated Neurofilament (pNF-H)

Packaging

96 wells

Preparation Note

Prior to use maintain the kit components at 2°-8°C and -20°C, as indicated, until expiration date.

1. Wash Buffer - After dilution maintain the Wash Solution at 2°-8°C for up to one month or until expiration date.

2. Tween^® 20 - Store at room temperature upon receipt of kit.

3. Elisa Assay Buffer - Aliquot as required to avoid repeated freeze-thaw cycles. After thawing, maintain the Elisa Assay Buffer at 2°-8°C for up to one month, or until expiration date.

4. Immunoplate Pre-coated with Chicken anti- pNF-H Polyclonal Antibody - Unused strip wells may be returned to the foil pouch and stored at 2°-8°C for up to one month, or until expiration date.

Other Notes

Immunoplate Pre-coated with Chicken anti-pNF-H Polyclonal Antibody: (Part No. 2006263). One 96-well immunoplate pre-coated with chicken anti- pNF-H polyclonal antibody sealed in a foil pouch.

Rabbit anti- pNF-H Polyclonal Antibody: (Part No. 2006919). One vial (100 μL) of rabbit anti- pNF-H polyclonal antibody.

Goat anti-Rabbit, Alkaline Phosphatase Conjugated Antibody: (Part No. 2006917). One vial (10 μL) of goat anti-rabbit alkaline phosphatase conjugate.

Bovine pNF-H Standard: (Part No. 2006918). One vial (5.0 ng) of bovine pNF-H standard.

20X TBS: (Part No. 2004913). One bottle (50 mL) of 20X TBS.

20% Tween^® 20: (Part No. 2004915). One vial (3 mL) of 20% Tween-20.

Elisa Assay Diluent: (Part No. 2006921) One bottle (30 mL) of Elisa assay diluent containg preservative, provided in a ready to use format.

pNPP Buffer: (Part No. 2006261). One bottle (12 mL) of pNPP buffer for use with 50X p-NPP, provided in a ready to use format.

50X pNPP: (Part No. 2006262). One vial (250 μL) of 50X pNPP (p-nitrophenyl phosphatase) alkaline phosphatase substrate.

Plate Covers - Two Plate Covers.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Disclaimer

For research use only. Not for use in diagnostic procedures.

Safety Information

• pictograms: GHS08,GHS05
• signalword: Danger
• hcodes: H315,H318,H361fd,H373
• pcodes: P202 - P260 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral
• target_organs: Kidney,Liver,Blood
• Storage Class: 10 - Combustible liquids
• wgk: WGK 3