GF092

TRAIL Protein, Recombinant human

Human TRAIL (TNF-Related Apoptosis Inducing Ligand), also called APO2 Ligand, is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells.

• Synonym(s): Apo2 Ligand
• UNSPSC Code: 12352202
• NACRES: NA.75
• eCl@ss: 32160405
• Form: solid
• Assay: >98% (SDS-PAGE and HPLC)
• Biological source: human
• Recombinant: expressed in E. coli
• Mol wt: 19.6 kDa

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• assay: >98% (SDS-PAGE and HPLC)
• form: solid
• mol wt: 19.6 kDa
• manufacturer/tradename: Chemicon^®
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 ng/μg Endotoxin (of TRAIL/Apo2L; 1EU/μg)
• input: sample type hematopoietic stem cell(s)
• suitability: suitable for molecular biology
• NCBI accession no.: NM_003810.2
• UniProt accession no.: P50591
• shipped in: dry ice
• Gene Information: human ... TNFSF10(8743)

Description

General description

Research area: CancerTNF-Related Apoptosis-Inducing Ligand (TRAIL) is a cytotoxic and type II membrane protein. It belongs to the tumor necrosis factor (TNF) superfamily. TRAIL is expressed by the cells of the immune system including natural killer cells, macrophages, T cells, and dendritic cells.[1] TRAIL Protein, Recombinant human is a 19.6 kDa protein

Application

TRAIL Protein, Recombinant human has been used as anapoptosis inducer: to extract cell-free DNA (cfDNA) from various cell lines[2]along with BV6 to study its anticancer effects in breast cancer cell lines[3], tostudy its effects of ethanol-induced interferon expression on TRAIL release in neurons[4]

Biochem/physiol Actions

TNF-Related Apoptosis-Inducing Ligand (TRAIL) promotes rapid apoptosis in tumor cells while sparing normal cells. Thisproperty of TRAIL makes it an important biotherapeutic agent in cancer therapy.It induces apoptosis by binding to two death signaling receptors, DR4 and DR5expressed on cell membranes.

Physical form

Sterile filtered, then lyophilized from 0.3 mg/mL in 10mM Sodium Phosphate + 110 mM NaCl.

Preparation Note

The lyophilized protein may be stored at room temperature for a few weeks, but is best stored at -20°C. After a quick spin, reconstitute in sterile water to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other buffered solutions and stored at 4ºC for 1 week or -20ºC for future use. Reconstituted GF092 should be stored in undiluted aliquots at -20°C for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3