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71235

pIEx-4 DNA - Novagen

Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Synonym(s):

InsectDirect™ System- pIEx-4 DNA

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About This Item

NACRES:
NA.51
UNSPSC Code:
41106611
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Quality Level

100

form

liquid

manufacturer/tradename

Novagen®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

General description

The pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.

pIEx-4 contains C-terminal S•Tag and His•Tag coding sequences. For sequencing or PCR of recombinants in pIEx-4, refer to the table below to choose the appropriate primers.

Legal Information

INSECTDIRECT is a trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the “EMD Product”) requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: [email protected].

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pIEx-4 DNA - Novagen Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Sigma-Aldrich

71235

pIEx-4 DNA - Novagen

pIEx-5 DNA - Novagen® Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Sigma-Aldrich

71242

pIEx-5 DNA - Novagen®

pIEx-10 DNA - Novagen® Novagen′s pIEx vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera derived insect cells.

Sigma-Aldrich

71557

pIEx-10 DNA - Novagen®

pBiEx-1 DNA - Novagen

Sigma-Aldrich

71234

pBiEx-1 DNA - Novagen

form

liquid

form

liquid

form

solid

form

liquid

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
71235-304055977271249

Questions

  1. What are the significant differences between vector pIEx-4 and pIEx-6? The aim is to express a membrane-specific protein without needing any signal peptidase at the 5' end.

    1 answer

    1. The pIEx™-4 DNA contains C-terminal S•Tag and His•Tag coding sequences, whereas the pIEx™-6 DNA contains an amino-terminal His•Tag coding sequence and an optional S•Tag coding sequence at the distal end of the multiple cloning site (MCS).

      Additionally, the pIEx™-6 DNA features a PshA I cloning site that overlaps the cleavage site for the enterokinase (EK) protease, enabling the removal of all amino-terminal vector-encoded sequences by EK digestion, which is not the case for pIEx™-4 DNA.

      Furthermore, neither pIEx™-4 DNA nor pIEx™-6 DNA offers the option of using a signal sequence to secrete the protein of interest into the surrounding medium.

      Helpful?

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