Key Documents

View All Documentation

HIV Reverse Transcriptase, Recombinant, E. coli

Name: HIV Reverse Transcriptase, Recombinant, <i>E. coli</i>
Brand: MM

liquid, ≥5,000 units/mg protein, Nuclease-free

Synonym(s):

HIV RT

Select a Size

Change View

Pack Size	SKU	Availability	Price

About This Item

NACRES:

NA.54

UNSPSC Code:

12352202

Form:

liquid

Recombinant:

expressed in E. coli

Pricing and availability is not currently available.

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

recombinant

expressed in E. coli

Quality Level

100

form

liquid

specific activity

≥10,000 units/mL, ≥5,000 units/mg protein

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

technique(s)

molecular cloning: suitable

input

purified RNA

foreign activity

Nuclease-free

shipped in

wet ice

storage temp.

−20°C

Description

General description

Recombinant HIV reverse transcriptase expressed in E. coli. Catalyzes error-prone synthesis on DNA and RNA templates. Provides an excellent target for evaluating antiviral agents.

Application

HIV Reverse Transcriptase, Recombinant, E.coli has been used as a standard in reverse transcriptase activity assay.[1]

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 10 mM potassium phosphate, 1 mM DTT, 20% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

One unit is defined as the amount of enzyme that will incorporate 1.0 nmol of labeled dTTP into acid-insoluble material in 20 min at 37°C, pH 8.3.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item	407294	616374	344145
Millipore 382129 HIV Reverse Transcriptase, Recombinant, E. coli	Sigma-Aldrich 407294 αA-Interferon, Human, Recombinant, E. coli	Sigma-Aldrich 616374 TRAIL, Human, Recombinant, E. coli	Sigma-Aldrich 344145 FPTase, Rat, Recombinant, E. coli
technique(s) molecular cloning: suitable	technique(s) cell based assay: suitable	technique(s) -	technique(s) -
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant expressed in E. coli
form liquid	form liquid	form liquid	form liquid
specific activity ≥10,000 units/mL, ≥5,000 units/mg protein	specific activity ≥2.8 x 10⁸ units/mg protein, ≥5 x 10⁶ units/mL	specific activity -	specific activity -
storage temp. −20°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
storage condition OK to freeze	storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles	storage condition OK to freeze, avoid repeated freeze/thaw cycles

Storage Class

10 - Combustible liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Conformation of HIV-1 Envelope Governs Rhesus CD4 Usage and Simian-Human Immunodeficiency Virus Replication.

Geraldine Vilmen et al.

mBio, e0275221-e0275221 (2022-01-12)

Infection of rhesus macaques with simian-human immunodeficiency viruses (SHIVs) is the preferred model system for vaccine development because SHIVs encode human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins (Envs)-a key target of HIV-1 neutralizing antibodies. Since the goal of vaccines

Identification of an antiretroviral small molecule that appears to be a host-targeting inhibitor of HIV-1 assembly

Reed JC, et al.

Journal of Virology, 95(3), 10-1128 (2021)

Conformation of HIV-1 Envelope governs rhesus CD4 usage and simian-human immunodeficiency virus replication

Vilmen G, et al.

mBio, 13(1), e02752-e02721 (2022)

View All Related Papers

Global Trade Item Number

SKU	GTIN
382129-500U	04055977213140

Questions

Reviews

No rating value