07-164

Anti-phospho-H2A.X (Ser139) Antibody

Upstate^®, from rabbit

• Synonym(s): H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human, vertebrates
• Application: ChIP, IP, WB
• Citations: 258

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, vertebrates
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable (ChIP-seq), immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_002096
• UniProt accession no.: P16104
• shipped in: ambient
• target post-translational modification: phosphorylation (pSer139)
• Gene Information: human ... HIST2H2AC(8338)

Description

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

15 kDa

Immunogen

Epitope: a.a. 134-142

Application

Anti-phospho-H2A.X (Ser139) Antibody is a Rabbit Polyclonal Antibody for detection of phospho-H2A.X (Ser139) also known as histone 2 H2ac, histone IIa & has been validated in IP & WB.

Immunoprecipitation:
A previous lot of this antibody successfully immunoprecipitated phosphorylated H2A.X as determined by an independent laboratory.

Biochem/physiol Actions

Broad species cross-reactivity expected based on conservation of sequence homology.

Histone H2A.X phosphorylated at Ser139.

Physical form

Format: Purified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Analysis Note

Control
Staurosporine-treated Jurkat lysate, UV-treated HT-29 cells, doxorubicin-treated HT-29 cells.

Routinely evaluated by Western Blot on untreated and staurosporine treated Jurkat lysate.

Western Blot Analysis:
1:500 dilution of this lot detected phospho-H2A.X on 10 μg of staurosporine treated Jurkat lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MABE205

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1