05-347

Anti-PCNA Antibody, clone PC10

clone PC10, Upstate^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: PC10, monoclonal
• Application: ICC, IHC, WB
• Citations: 21

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: PC10, monoclonal
• species reactivity: vertebrates
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NM_002592
• UniProt accession no.: P12004
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PCNA(5111)

Description

General description

36kDa

Expression of proliferating cell nuclear antigen (PCNA), cyclin or polymerase delta auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-delta to play a fundamental role in the initiation of cell proliferation. Anti-PCNA reacts with PCNA at the molecular weight of 36 kDa on western blot.

Immunogen

Recombinant rat PCNA

Application

Detect PCNA with Anti-PCNA Antibody, clone PC10 (Mouse Monoclonal Antibody), that has been shown to work in WB, ICC & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

PCNA

Physical form

Concentrated culture supernatant

Format: Purified

Protein A purified mouse immunoglobulin in PBS containing 1 mg/mL BSA and 10mM Sodium Azide.

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

routinely evaluated by immunoblot on RIPA lysate from human A431 cells or mouse 3T3/A31 cell lysate

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable