04-824

Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal

culture supernatant, clone 68-4, from rabbit

• Synonym(s): Estradiol receptor, Nuclear receptor subfamily, estrogen receptor 1, estrogen receptor alpha
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 68-4, monoclonal
• Application: IP, WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 68-4, monoclonal
• species reactivity: mouse, rat, human
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_001437
• UniProt accession no.: P03372
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... ESR2(2100) ; mouse ... Esr2(13983) ; rat ... Esr2(25149)

Description

General description

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics.

59kDa

Immunogen

Epitope: a.a.55-74(H) or 63-82 (R, M)

KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β.

Application

Detect Estrogen Receptor β using this Anti-Estrogen Receptor β Antibody, clone 68-4 validated for use in WB & IP.

Immunoprecipitation:
10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate.

Biochem/physiol Actions

Estrogen receptor β, long form, does not detect estrogen receptor alpha.

Predicted to cross-react with mouse and rat based on sequence homology.

Analysis Note

Evaluated by western blot on RIPA lysates from MCF-7 cells.

Western Blot Analysis:
A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable