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HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)

HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1) application for HPLC

Materiali

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

T1021

Inibitore della tripsina

Isoelectric focusing marker, pI 4.6

T9767

Inibitore della tripsina

For use as a marker in SDS-PAGE, BioReagent

T9003

Inibitore della tripsina

lyophilized powder

T9128

Inibitore della tripsina

lyophilized powder

T6522

Inibitore della tripsina

powder, BioReagent, suitable for cell culture

M1882

Myoglobin from equine heart

≥90% (SDS-PAGE), essentially salt-free, lyophilized powder

M9267

Myoglobin from equine heart

Isoelectric focusing marker, pI (1) 6.8, (2) 7.2

M6036

Myoglobin from human heart

≥95% (SDS-PAGE), buffered aqueous glycerol solution

T0256

Trypsin inhibitor from bovine pancreas

Type I-P, essentially salt-free, lyophilized powder

C0880

Conalbumin from chicken egg white

Iron complex

C7786

Conalbumin from chicken egg white

BioReagent, suitable for cell culture

C0755

Conalbumin from chicken egg white

Substantially iron-free

T9378

Trypsin inhibitor from Phaseolus limensis (lima bean)

Type II-L, crude powder

T6414

Inibitore della tripsina

solution, sterile-filtered, suitable for, suitable for cell culture

M0630

Myoglobin from equine skeletal muscle

95-100%, essentially salt-free, lyophilized powder

T2011

Trypsin inhibitor from chicken egg white

Type III-O (free of ovoinhibitor)

T9253

Trypsin inhibitor from chicken egg white

Type II-O, Partially purified ovomucoid, containing ovoinhibitor

93620

Inibitore della tripsina

lyophilized powder, ~10000 U/mg

M5696

Myoglobin from equine skeletal muscle

BioUltra, 95-100% (SDS-PAGE)

T2327

Trypsin Inhibitor from Glycine max (soybean)

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

CONDITIONS

column

Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)

mobile phase

[A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)

flow rate

0.5 mL/min

detector

UV, 280 nm

injection

10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A

Descrizione

Risultati analitici

The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.

Note legali

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS