T9378

Trypsin inhibitor

crude powder

• Sinonimo/i: Trypsin Inhibitor from lima beans
• Numero CAS: 9035-81-8
• Numero CE: 232-906-9
• Numero MDL: MFCD00082102
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• Origine biologica: Phaseolus limensis (lima bean)
• Livello qualitativo: 200
• Tipo: Type II-L
• Stato: crude powder
• PM: 9 kDa
• Solubilità: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• Temperatura di conservazione: 2-8°C

Descrizione

Applicazioni

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma[1]
• in the termination of proteolytic digestion in rabbit skeletal muscles[2]
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris[3]
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract[4]

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type[5] enzymes.

Azioni biochim/fisiol

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors.[6] It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.[7]

Proprietà fisiche

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definizione di unità

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota sulla preparazione

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Risultati analitici

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Altre note

View more information on Trypsin Inhibitor.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H317,H334
• Consigli di prudenza: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classi di pericolo: Resp. Sens. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)