Il terreno RPMI 1640 è stato sviluppato nel 1966 presso il Roswell Park Memorial Institute da Moore e i suoi collaboratori. Inizialmente ottenuto da una modifica del terreno 5A di McCoy per supportare la crescita di cellule linfoblastoidi in sospensione, si è visto successivamente che è in grado di supportare anche la crescita di un′ampia varietà di cellule che dipendono dall′adesione. Inizialmente ideato per essere supplementato con siero, l′RPMI 1640 si è dimostrato in grado di supportare la crescita di diverse linee cellulari anche in assenza di siero. È anche ampiamente usato in protocolli di fusione e per la crescita di cellule ibride. Questo terreno è adatto per la coltura di leucociti umani normali e neoplastici.
Altre note
Modified for autoclaving Without L-glutamine and sodium bicarbonate.
Quantità
Formulated to contain 10.3 grams of powder per liter of medium.
Ricostituzione
Supplement with 0.3 L-glutamine, 2.0 g/L sodium bicarbonate.
Inhibition of fungal growth by Congo red (CR) has been putatively associated with specific binding to β-1,3-glucans, which blocks cell wall polysaccharide synthesis. In this study, we searched for transcription factors (TFs) that regulate the response to CR and interrogated
Frontiers in cellular and infection microbiology, 11, 637836-637836 (2021-09-14)
The highly conserved, ubiquitous molecular chaperone Hsp90 is a key regulator of cellular proteostasis and environmental stress responses. In human pathogenic fungi, which kill more than 1.6 million patients each year worldwide, Hsp90 governs cellular morphogenesis, drug resistance, and virulence.
In the poultry industry, quantitative analysis of chicken T cell proliferation is important in many biological applications such as drug screening, vaccine production, and cytotoxicity assessment. Several assays have been established to evaluate this immunological response in chicken cells. However
Leukocyte adhesion in retinal microvasuculature substantially contributes to diabetic retinopathy. Involvement of the Rho/Rho kinase (ROCK) pathway in diabetic microvasculopathy and therapeutic potential of fasudil, a selective ROCK inhibitor, are investigated. Localization of RhoA/ROCK and Rho activity were examined in
BMC complementary and alternative medicine, 11, 58-58 (2011-07-27)
Mushroom polysaccharides have traditionally been used for the prevention and treatment of a multitude of disorders like infectious illnesses, cancers and various autoimmune diseases. Crude mushroom extracts have been tested without detailed chemical analyses of its polysaccharide content. For the
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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