Ugrás a tartalomra
Merck

581324-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

Szinonimák:

Ascentis RP18 HPLC Column

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm

material

stainless steel column

Minőségi szint

Ügynökség

suitable for USP L1 (Similar to Phenomenex Luna C18)

termékcsalád

Ascentis®

Jellemzők

endcapped

gyártó/kereskedő neve

Ascentis®

kiszerelés

1 ea of

a címkézés mértéke

25% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technika/technikák

HPLC: suitable
LC/MS: suitable

H × belső átmérő

15 cm × 4.6 mm

külső felület

450 m2/g

felület bevonatolás

3.7 μmol/m2

szennyeződések

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

mátrix aktív csoport

C18 (octadecyl) phase

részecskeméret

5 μm

pórusméret

100 Å

operating pH range

2-8

alkalmazás(ok)

food and beverages

elválasztástechnika

reversed phase

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Általános leírás

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Alkalmazás


  • Preparation of polar embedded C18 stationary phase for efficient separation of peptides and proteins in high performance liquid chromatography.: This study details the development of a polar embedded C18 stationary phase, demonstrating its effectiveness in separating peptides and proteins. The Ascentis® C18 HPLC Column was utilized for its high separation efficiency, crucial for proteomics and biopharmaceutical applications (Ali et al., 2022).

  • A dilute-and-shoot liquid chromatography-tandem mass spectrometry method for urinary 18-hydroxycortisol quantification and its application in establishing reference intervals.: Researchers used the Ascentis® C18 HPLC Column to develop a sensitive method for quantifying urinary 18-hydroxycortisol. This method aids in clinical diagnostics and endocrinological studies by providing accurate reference intervals (Zhao et al., 2022).

  • A Comparative Study on Analysis of Ginsenosides in American Ginseng Root Residue by HPLC-DAD-ESI-MS and UPLC-HRMS-MS/MS.: This comparative analysis utilized the Ascentis® C18 HPLC Column to separate and identify ginsenosides in American ginseng. The study highlights the column′s application in natural product research and quality control in the pharmaceutical industry (Hsu et al., 2022).

  • Validation of a Rapid and Easy-to-Apply Method to Simultaneously Quantify Co-Loaded Dexamethasone and Melatonin PLGA Microspheres by HPLC-UV: Encapsulation Efficiency and In Vitro Release.: This research validates an HPLC method using the Ascentis® C18 Column to simultaneously quantify dexamethasone and melatonin in PLGA microspheres, offering a robust tool for drug delivery studies (Brugnera et al., 2022).

  • Simultaneous determination of ivermectin, clorsulon and their related substances in an injectable finished product by a stability-indicating RP-HPLC method.: The Ascentis® C18 HPLC Column was employed to develop a stability-indicating method for analyzing ivermectin and clorsulon in pharmaceutical formulations, emphasizing its role in quality control and regulatory compliance (Padivitage et al., 2022).

Tulajdonságok és előnyök

  • Excellent retention
  • Symmetric peak shape
  • High reproducibility
  • Complete LC-MS compatibility

Javasolt termékek

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Jogi információk

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

Dipanjan Goswami et al.
Biomedical chromatography : BMC, 23(11), 1227-1241 (2009-07-14)
A LC-MS/MS method for plasma topiramate analysis is delineated involving least number of healthy volunteers. Topiramate and amlodipine internal standard (IS) were extracted by simple centrifuge-coupled solid-phase extraction and reverse-phase chromatographic separation was performed on an Ascentis C(18) column. Turbo-spray
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 55(5), 934-948 (2011-04-19)
In this study, the composition of polyphenols (phenolic acids and flavonoids) in propolis extracts was investigated by HPLC-DAD and HPLC-ESI-MS/MS by comparing the performance of ion trap and triple quadrupole mass analyzers. The analyses were carried out on an Ascentis
Federica Vacondio et al.
Journal of pharmaceutical and biomedical analysis, 46(1), 200-205 (2007-10-26)
A rapid, simple and sensitive liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of the imidazole H(3) antagonist ROS203 in rat plasma, using the superior homologue ROS287 as internal standard. Analyses were performed on an Agilent
Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
Federica Pellati et al.
Journal of chromatography. A, 1242, 43-58 (2012-05-09)
In this study, a detailed phytochemical characterization of Echinacea pallida (Nutt.) Nutt. root extracts and dietary supplements was carried out for the first time by developing advanced chromatographic techniques, based on HPLC with diode array (DAD) and electrospray ionization-mass spectrometry

Cikkek

Phosphonates analysis challenges due to their polar nature and lack of UV active chromophores in antiviral drugs.

Protocols

Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.

HPLC Analysis of Warfarin™ Anticoagulant on Ascentis® C18

Chromatograms

application for HPLCapplication for HPLCapplication for HPLCapplication for HPLC

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