T9650

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

• Szinonimák: TAE buffer
• MDL-szám: MFCD00236357
• UNSPSC kód: 41105319
• PubChem Substance ID: 329826908
• NACRES: NA.25

Tulajdonságok

• grade: for molecular biology
• Minőségi szint: 200
• sterilitás: non-sterile; 0.2 μm filtered
• termékcsalád: BioReagent
• form: solution
• alkalmasság: suitable for gel electrophoresis (after dilution to working concentration)
• idegen aktivitás: Protease, none detected; RNAse, none detected
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• Nemzetközi kémiai azonosító kulcs: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Leírás

Alkalmazás

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Egyéb megjegyzések

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Elkészítési megjegyzés

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Solution prepared with 18 megohm water

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H373
• Óvintézkedésre vonatkozó mondatok: P260 - P313 - P501
• Veszélyességi osztályok: STOT RE 2 Inhalation
• Célzott szervek: Respiratory Tract
• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable