T4038

Tris Acetate-EDTA buffer

BioReagent, DNase and RNase, none detected, suitable for electrophoresis, 10× concentrate

• Szinonimák: TAE buffer
• MDL-szám: MFCD00236357
• UNSPSC kód: 41105319
• PubChem Substance ID: 329826782
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 200
• termékcsalád: BioReagent
• form: powder blend
• koncentráció: 8.5-10.5 ×
• szennyeződések: DNase and RNase, none detected
• pH: 8.1-8.5
• oldhatóság: water: 64.2 g/L, clear, colorless
• alkalmasság: suitable for electrophoresis; suitable for gel electrophoresis (after dilution to working concentration)
• alkalmazás(ok): diagnostic assay manufacturing
• SMILES string: CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
• Nemzetközi kémiai azonosító kulcs: HGEVZDLYZYVYHD-UHFFFAOYSA-N

Leírás

Alkalmazás

Ready for use in gel electrophoresis after dilution to working concentrations.

TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Kiszerelés

Packaged in pouches

Elkészítési megjegyzés

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).

Feloldás

Produces a 10× concentrate (0.4 M Tris-acetate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Warning
• Figyelmeztető mondatok: H373
• Óvintézkedésre vonatkozó mondatok: P260 - P314 - P501
• Veszélyességi osztályok: STOT RE 2 Inhalation
• Célzott szervek: Respiratory Tract
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: dust mask type N95 (US), Eyeshields, Gloves