T1952

Trichostatin A, Ready Made Solution

5 mM in DMSO, from Streptomyces sp.

• Szinonimák: TSA, [R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide
• Tapasztalati képlet (Hill-képlet): C₁₇H₂₂N₂O₃
• CAS-szám: 58880-19-6
• Molekulatömeg: 302.37
• UNSPSC kód: 12352202
• NACRES: NA.32

Tulajdonságok

• biológiai forrás: Streptomyces sp.
• Minőségi szint: 200
• Teszt: ≥98% (HPLC)
• form: DMSO solution
• koncentráció: 5 mM in DMSO
• technika/technikák: cell culture | mammalian: suitable
• antibiotikus hatásspektrum: fungi; neoplastics
• Hatásmechanizmus: enzyme | inhibits
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• InChI: 1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
• Nemzetközi kémiai azonosító kulcs: RTKIYFITIVXBLE-WKWSCTOISA-N

Leírás

Általános leírás

Trichostatin A is a compound of primary hydroxamic acid.

Alkalmazás

Trichostatin A, ready-made solution has been used:

• as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
• to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
• to treat cells for the HDAC inhibition experiments

Biokémiai/fiziológiai hatások

Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)