Összes fotó(3)
Fontos dokumentumok
P9136
Pyruvate Kinase from rabbit muscle
Type III, lyophilized powder, 350-600 units/mg protein
Szinonimák:
ATP:pyruvate 2-O-phosphotransferase, PK
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
Javasolt termékek
biológiai forrás
rabbit muscle
Minőségi szint
típus
Type III
Forma
lyophilized powder
specifikus aktivitás
350-600 units/mg protein
molekulatömeg
237 kDa
idegen aktivitás
lactic dehydrogenase, creatine phosphokinase, phosphoglucomutase, and myokinase ≤0.01%
tárolási hőmérséklet
−20°C
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Általános leírás
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.
Alkalmazás
Pyruvate Kinase from rabbit muscle has been used to convert adenosine diphosphate (ADP) to adenosine triphosphate (ATP) in Pseudomonas putida cells.
Pyruvate kinase has been used in plant spectrophotometric assays to measure ATP hydrolysis . Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency .
Biokémiai/fiziológiai hatások
Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase (PK) catalyzes an important process of transferring phosphate group from phosphoenolpyruvate (PEP) to adenosine diphosphate (ADP). This reaction results in the conversion of PEP to pyruvate and adenosine triphosphate (ATP). Pyruvate kinase plays a major role in glycolysis and gluconeogenesis. High levels of pyruvate kinase M2 (PKM2) inhibits cell proliferation and tumor growth. Deficiency of PK in red blood cells (RBC) leads to non-spherocytic hemolytic anaemia. In human erythrocytes, deficiency of PK induces a protective effect against Plasmodium falciparum.
Egység definíció
One unit will convert 1.0 μmole of phospho(enol)pyruvate to pyruvate per min at pH 7.6 at 37 °C.
Analízis megjegyzés
Protein determined by biuret.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Az ügyfelek ezeket is megtekintették
Red cell pyruvate kinase deficiency: molecular and clinical aspects
Zanella A, et al.
British Journal of Haematology, 130(1), 11-25 (2005)
Anoxic metabolism and biochemical production in Pseudomonas putida F1 driven by a bioelectrochemical system
Lai B, et al.
Biotechnology for Biofuels, 9(1), 39-39 (2016)
[25] Pyruvate kinase isozymes from rat
Methods in Enzymology, 90, 150-165 (1982)
Pyruvate kinase deficiency and malaria
Ayi K, et al.
The New England Journal of Medicine, 358(17), 1805-1810 (2008)
Hormonal control of pyruvate kinase activity and of gluconeogenesis in isolated hepatocytes
Feliu J E, et al.
Proceedings of the National Academy of Sciences of the USA, 73(8), 2762-2766 (1976)
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