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P4056

Sigma-Aldrich

Platelet-Derived Growth Factor-BB from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Szinonimák:

PDGF-BB

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

MDL-szám:
MFCD01094933
UNSPSC kód:
12352202
NACRES:
NA.77

product name

Platelet-Derived Growth Factor-BB from rat, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

rekombináns

expressed in E. coli

Minőségi szint

100

form

lyophilized powder

hatékonyság

1.62 ng/mL EC50

minőség

endotoxin tested

molekulatömeg

~30 kDa

kiszerelés

pkg of 5x50 μg
pkg of 50 μg

tárolási körülmény

avoid repeated freeze/thaw cycles

technika/technikák

cell culture | mammalian: suitable

szennyeződések

≤1 EU/μg

UniProt elérési szám

P31240

tárolási hőmérséklet

−20°C

Géninformáció

rat ... Pdgfb(24628)

Related Categories

Általános leírás

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

Alkalmazás

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

Biokémiai/fiziológiai hatások

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Egyéb megjegyzések

Dimer of the B chain of rat PDGF.

Fizikai forma

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Analízis megjegyzés

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Eileen M Bauer et al.
PloS one, 9(5), e96720-e96720 (2014-05-20)
Pulmonary hypertension (PH) is a progressive and fatal disease with no cure. Vascular remodeling in PH involves intraluminal growth of endothelial and smooth muscle cells, leading to obliterative vascular lesions. Cell growth in these lesions is quasi-neoplastic, with evidence of
Skeletal muscle tissue engineering: A maturation model promoting long-term survival of myotubes, structural development of the excitation?contraction coupling apparatus and neonatal myosin heavy chain expression.
Das M, et al.
Biomaterials, 30.29, 5392-5402 (2009)
Xin Zhou et al.
Current protocols, 2(5), e416-e416 (2022-05-10)
The serine/threonine protein kinase Akt integrates diverse upstream inputs to regulate cell survival, growth, metabolism, migration, and differentiation. Mounting evidence suggests that Akt activity is differentially regulated depending on its subcellular location, which can include the plasma membrane, endomembrane, and
Platelet-Derived Growth Factor?BB Transactivates the Fibroblast Growth Factor Receptor to Induce Proliferation in Human Smooth Muscle Cells.
Millette E, et al.
Trends in Cardiovascular Medicine, 16.1, 25-28 (2006)
Intraglomerular expressions of IL-1a and platelet-derived growth factor (PDGF-B) mRNA in experimental immune complex-mediated glomerulonephritis.
Akai Y, et al.
Clinical and Experimental Immunology, 95.1, 29-34 (1994)
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Cikkek

Platelet Derived Growth Factors (PDGF)

Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.

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