P4056

Platelet-Derived Growth Factor-BB from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Szinonimák: PDGF-BB
• MDL-szám: MFCD01094933
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• Terméknév: Platelet-Derived Growth Factor-BB from rat, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• rekombináns: expressed in E. coli
• Minőségi szint: 100
• Forma: lyophilized powder
• hatékonyság: 1.62 ng/mL EC₅₀
• minőség: endotoxin tested
• molekulatömeg: ~30 kDa
• kiszerelés: pkg of 5x50 μg; pkg of 50 μg
• tárolási körülmény: avoid repeated freeze/thaw cycles
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: ≤1 EU/μg
• UniProt elérési szám: P31240
• tárolási hőmérséklet: −20°C
• Géninformáció: rat ... Pdgfb(24628)

Leírás

Általános leírás

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B.[1][2] PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.[3]

Alkalmazás

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.[4][3]

Biokémiai/fiziológiai hatások

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes.[5] PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts.[6] PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation.[7] It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

Egyéb megjegyzések

Dimer of the B chain of rat PDGF.

Fizikai forma

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

Analízis megjegyzés

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)