11284932001
Roche
DNase I
from bovine pancreas
Szinonimák:
Dnase
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
biológiai forrás
bovine pancreas
Minőségi szint
form
lyophilized
kiszerelés
pkg of 100 mg
gyártó/kereskedő neve
Roche
technika/technikák
cell culture | mammalian: suitable
tárolási hőmérséklet
2-8°C
Általános leírás
DNase I is a double-strand specific endonuclease that degrades DNA. Bovine pancreatic deoxyribonuclease I (DNase I) is a DNA minor grove-interacting nuclease, which shows relatively low specificity. This protein is composed of two central β sheets, each composed of six β-strands. This structure is surrounded by extensive loop and α-helical regions. This enzyme shares structural similarity to exonuclease III. DNase I is one of the most well characterized endonucleases of mammalian origin.
Alkalmazás
DNase I has been used for tissue dissociation in conjunction with collagenases.
Biokémiai/fiziológiai hatások
During tissue dissociation, parts of the cells are lysed resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, therby avoiding the loss of cells from undesired clumping.
Tulajdonságok és előnyök
- Lyophilized
- Filtered through 0.2 μm pore size membrane before lyophilization
- Glycoprotein and double-strand-specific endonuclease
Egység definíció
One unit is the enzyme activity which yields an increase in absorbance of 0.001 per minute under the assay conditions.
Elkészítési megjegyzés
Activator: Bivalent metal ions (Ca2+, Mg2+)
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Working concentration: 0.01 to 1 mg/ml
Note: For each cell type the working concentration has to be determined individually. For optimal activity the enzyme needs 5 mM Mg2+.
Feloldás
Reconstitute the lyophylizate in sterile double-distilled water (10 mg/ml); further dilution with PBS (phosphate buffered saline), HBSS (Hank′s Balanced Salt Solution) or medium.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Resp. Sens. 1 - Skin Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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