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Merck
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Fontos dokumentumok

GF149

Sigma-Aldrich

PDGF-BB, recombinant human

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

Szinonimák:

Platelet Derived Growth Factor-BB

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352202
eCl@ss:
32160405
NACRES:
NA.77

biológiai forrás

human

Minőségi szint

Teszt

>98% (SDS-PAGE)

gyártó/kereskedő neve

Chemicon®

technika/technikák

cell culture | mammalian: suitable

szennyeződések

<0.1 ng/μg endotoxin (of PDGF-BB; 1EU/μg)

bemenet

sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)

UniProt elérési szám

kiszállítva

dry ice

Géninformáció

human ... PDGFB(5155)

Általános leírás

Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
Product Source: E. coli

Alkalmazás

For most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.
The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

Kapcsolódás

Replaces: GF018

Fizikai forma

Sterile filtered solution lyophilized from 10 mM HOAc.
The sterile filtered solution was lyophilized from 10 mM HOAc.

Tárolás és stabilitás

The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.

Analízis megjegyzés

Specific Activity: Human PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.

Jogi információk

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Karla Lucia F Alvarez et al.
STAR protocols, 3(1), 101149-101149 (2022-02-11)
Here, we present a protocol to culture primary human vascular smooth muscle cells (VSMCs) under Alzheimer's disease (AD)-like conditions, including steps for morphological characterization with microscopy. We then describe functional assays, including wound healing, transwell, coculture, and supernatant assays, to
Yi Ma et al.
Journal of cellular and molecular medicine, 25(11), 5202-5219 (2021-05-05)
Pulmonary arterial hypertension (PAH) featured a debilitating progressive disorder. Here, we intend to determine diagnosis-valuable biomarkers for PAH and decode the fundamental mechanisms of the biological function of these markers. Two mRNA microarray profiles (GSE70456 and GSE117261) and two microRNA
Xunde Xian et al.
eLife, 6 (2017-11-17)
Low-density lipoprotein receptor-related protein 1 (LRP1) is a multifunctional cell surface receptor with diverse physiological roles, ranging from cellular uptake of lipoproteins and other cargo by endocytosis to sensor of the extracellular environment and integrator of a wide range of
Zili Zhang et al.
BioFactors (Oxford, England), 47(5), 801-818 (2021-06-16)
Targeting the elimination of activated hepatic stellate cells (HSCs) and blocking excessive deposition of extracellular matrix are recognized as an effective strategy for the treatment of hepatic fibrosis. As a newly discovered programmed cell death mode, the regulatory mechanism of
Lifeng Jiang et al.
Frontiers in medicine, 9, 886868-886868 (2022-07-16)
Hyper-proliferation of pulmonary arterial vascular smooth muscle cells (PAVSMC) is an important pathological component of pulmonary vascular remodeling in pulmonary arterial hypertension (PAH). Lipogenesis is linked to numerous proliferative diseases, but its role in PAVSMC proliferation in PAH remains to

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