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G8885

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type H-3, aqueous solution, ≥90,000 units/mL

Szinonimák:

β-D-Glucuronide glucuronosohydrolase

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)

About This Item

CAS-szám:
9001-45-0
Enzyme Commission szám:
3.2.1.31 (BRENDA, IUBMB)
MDL-szám:
MFCD00130629
UNSPSC kód:
12352204
NACRES:
NA.54

típus

Type H-3

Minőségi szint

200

Forma

aqueous solution

specifikus aktivitás

≥90,000 units/mL

secondary activity

≤1,000 units/mL sulfatase

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

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Általános leírás

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Alkalmazás

β-Glucuronidase from Helix pomatia has been used:
  • to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection
  • as a component in the protoplasting solution
  • for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)
  • to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples

β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo.
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biokémiai/fiziológiai hatások

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Minőség

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Egység definíció

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Fizikai forma

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

szubsztrát

Product No.
Leírás
Árazás

Piktogramok

Health hazard
GHS08

Figyelmeztetés

Danger

Figyelmeztető mondatok

H334

Óvintézkedésre vonatkozó mondatok

P261 - P284 - P501

Veszélyességi osztályok

Resp. Sens. 1

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

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Analitikai tanúsítványok (COA)

Lot/Batch Number
0000411984
0000411988
0000411987
0000411988
0000411987

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Dokumentumtár megtekintése

Equol producer status, salivary estradiol profile and urinary excretion of isoflavones in Irish Caucasian women, following ingestion of soymilk
Hall MC, et al.
Steroids, 72(1), 64-70 (2007)
S Jacobs et al.
Journal of enzyme inhibition, 4(4), 315-325 (1991-01-01)
By modification of a recently developed method for separation of radio-labelled urinary oestrogens we were able to separate oestrogen metabolites and measure their isotope ratios in urine following injections of [3H]delta 4-androstenedione and [14C]oestrone. This method provides a useful tool
The zrfA and zrfB genes of Aspergillus fumigatus encode the zinc transporter proteins of a zinc uptake system induced in an acid, zinc-depleted environment
Vicentefranqueira R, et al.
Eukaryotic Cell, 4(5), 837-848 (2005)
A metabolite profiling approach to identify biomarkers of flavonoid intake in humans
Loke WM, et al.
The Journal of Nutrition, 139(12), 2309-2314 (2009)
Andrew M Jenner et al.
Free radical biology & medicine, 38(6), 763-772 (2005-02-22)
Phenolic compounds are not completely absorbed in the small intestine and so enter the colon, where they might exert physiological effects. To identify phenolics that are present in normal human colon, fecal water was prepared from 5 free-living volunteers with
View All Related Papers

Cikkek

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Protocols

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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