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Merck

D2906

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, sodium pyruvate and HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Szinonimák:

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and 15 mM HEPES, without sodium bicarbonate and phenol red, powder, suitable for cell culture

Minőségi szint

form

powder

technika/technikák

cell culture | mammalian: suitable

alkatrészek

NaHCO3: no
L-glutamine: yes
phenol red: no
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L
HEPES: 15 mM

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

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Általános leírás

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is suitable for the growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.

Alkalmazás

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • Hep-2 cells
  • mouse fibroblast L‐cells
  • L‐ Wnt family member 3A (Wnt3a) cells
  • primary buffalo embryonic fibroblast (BEF)

Mennyiség

Formulated to contain 15.6 grams of powder per liter of medium.

Feloldás

Supplement with 1.2 g/L sodium bicarbonate.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

C Zhang et al.
Osteoarthritis and cartilage, 26(6), 824-833 (2018-03-20)
Adipokines play roles in the pathogenesis of osteoarthritis (OA). Fatty acid binding protein 4 (FABP4) is a novel adipokine that is closely associated with obesity and metabolic diseases. The aim of this study was to discover the potential role of
Eric M Erkenbrack et al.
PLoS biology, 16(8), e2005594-e2005594 (2018-08-25)
Among animal species, cell types vary greatly in terms of number and kind. The number of cell types found within an organism differs considerably between species, and cell type diversity is a significant contributor to differences in organismal structure and
Nereo Kalebic et al.
EMBO reports, 17(3), 338-348 (2016-01-14)
We have applied the CRISPR/Cas9 system in vivo to disrupt gene expression in neural stem cells in the developing mammalian brain. Two days after in utero electroporation of a single plasmid encoding Cas9 and an appropriate guide RNA (gRNA) into
Tao Fu et al.
FEBS letters, 593(15), 2040-2050 (2019-06-04)
Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed
Peter S B Finnie et al.
Current biology : CB, 30(10), 1934-1940 (2020-04-04)
Memory allows organisms to predict future events based on their prior sampling of the world. Rather than faithfully encoding each detail of related episodes, the brain is thought to incrementally construct probabilistic estimates of environmental statistics that are re-evaluated each

Cikkek

Nutrient Mixture F-12 Ham Formulation

Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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