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Merck
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F7524

Sigma-Aldrich

Fetal Bovine Serum

Fetal Bovine Serum
1 of 8 reviewers received a sample product or took part in a promotion

non-USA origin, sterile-filtered, suitable for cell culture

Szinonimák:

Fetal Calf sera, Fetal Calf serum, FBS, FCS, sera, serum

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

50 ML
inquire
100 ML
inquire
500 ML
inquire

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Összes fotó(1)

Méret kiválasztása

Nézet módosítása
50 ML
inquire
100 ML
inquire
500 ML
inquire

About This Item

MDL-szám:
MFCD00132239
UNSPSC kód:
12352207
eCl@ss:
32160409
NACRES:
NA.71
biológiai forrás:
bovine fetus
eredet:
non-USA origin
sterilitás:
sterile-filtered
kiszállítva:
dry ice

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.

biológiai forrás

bovine fetus

Minőségi szint

400

sterilitás

sterile-filtered

termékcsalád

FBS Classic

összetétel

hemoglobin, ≤25 mg/dL

eredet

non-USA origin

elérhetőség

not available in Canada, USA

technika/technikák

cell culture | mammalian: suitable

szennyeződések

≤10 EU/mL endotoxin

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

Looking for similar products? Látogasson el ide Útmutató a termékösszehasonlításhoz

Alkalmazás

Fetal Bovine Serum has been used as a supplement in cell culture media such as Roswell Park Memorial Institute medium (RPMI-1640) and Dulbecco′s Modified Eagles Media (DMEM)[1]to support the growth of a variety of cell lines.

Biokémiai/fiziológiai hatások

Fetal bovine serum (FBS) serves as a commonly utilized cell culture media supplement to support in vitro and ex vivo cell, tissue, and organ cultures. FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth, and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It provides many non-defined growth-promoting and survival enhancing factors to cells in culture. Derived from clotted blood, it is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. FBS is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Tulajdonságok és előnyök

F7524 is the same great product as Biochrom GmbH. product S0115.

Analízis megjegyzés

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Egyéb megjegyzések

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Jogi információk

Not available in USA or Canada. Please check with your local supplier regarding availability and import restrictions in your country.

gyakran vásárolják ezzel a termékkel együtt

Product No.
Leírás
Árazás

javasolt

Product No.
Leírás
Árazás

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0001687519
0001675653
0001677192
0001687014
0001689071

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Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.

COA keresése

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Elisabeth F P Peterse et al.
BMC cancer, 16, 475-475 (2016-07-16)
Chondrosarcoma is a malignant cartilage forming bone tumour for which no effective systemic treatment is available. Previous studies illustrate the need for a better understanding of the role of the IGF pathway in chondrosarcoma to determine if it can be
Hans F N Kvitvang et al.
Journal of chromatography. A, 1370, 70-79 (2014-12-03)
Metabolic profiling has become an important tool in biological research, and the chromatographic separation of metabolites coupled with mass spectrometric detection is the most frequently used approach for such studies. The establishment of robust chromatographic methods for comprehensive coverage of
Carlo E A Jochems et al.
Alternatives to laboratory animals : ATLA, 30(2), 219-227 (2002-04-25)
Fetal bovine serum (FBS) is a common component of animal cell culture media. It is harvested from bovine fetuses taken from pregnant cows during slaughter. FBS is commonly harvested by means of a cardiac puncture without any form of anaesthesia.
Michelle J Nyhan et al.
BMC cancer, 16, 101-101 (2016-02-18)
Successful treatment of oesophageal cancer is hampered by recurrent drug resistant disease. We have previously demonstrated the importance of apoptosis and autophagy for the recovery of oesophageal cancer cells following drug treatment. When apoptosis (with autophagy) is induced, these cells
Maria Peiris-Pagès et al.
Aging, 11(14), 4801-4835 (2019-07-18)
Using proteomics analysis, we previously compared MCF7 breast cancer cells grown as 3D tumor spheres, with the same cell line grown as monolayers. Our results indicated that during 3D anchorage-independent growth, the cellular machinery associated with i) mitochondrial biogenesis and
View All Related Papers

Cikkek

Fetal Bovine Serum (FBS) – What You Need to Know

Review the basics of using fetal bovine (or calf) serum for cell culture and how sourcing and regulations affect the price of sera for cell-based assays.

Related Content

Fetal Bovine Serum that Makes the Grade

Explore our portfolio of fetal bovine serum (FBS) and determine the right FBS for your lab needs and budget. Request a serum sample.

8 Reviews This action will navigate to reviews.

Questions

1–10 of 16 Questions  

  1. When aliquioting FBS, is it best to cool them to 4ºC first or directly at -80ºC

    1 answer

    1. The serum can be aliquoted into smaller, single-use portions and refrozen directly.

      Helpful?

  2. Hello, I have a question regarding FBS (F7524), for my experiment it is essential there is no dopamine or serotonine in my medium. Is it known wether these substances are present in FBS?

    1 answer

    1. This product has not undergone testing to determine the presence of dopamine or serotonin.

      Helpful?

  3. Could the presence of insoluble precipitates when thawing the serum indicate contamination?

    1 answer

    1. ​Thawed serum may contain precipitates and flocculants due to various reasons, such as residual fibrinogen converting to fibrin upon thawing. Centrifugation at 400 xg can remove excess fibrin, while filtering serum is not recommended to avoid protein depletion and clogged filters.
      Additional points to consider:
      •Cloudiness in human serum products is common and indicates the presence of lipids. Clear, lipid-free human serum products are not available.
      •Microscopic serum precipitates may resemble yeast contamination but are actually lipid aggregates that can be dissolved by lipase. They do not show growth in culture or gram staining and can be removed through centrifugation or filtration.
      •Gel layer or clumps in serum are caused by poor or overheated inactivation or improper mixing of FBS during the Heat inactivation process. Serum with these characteristics should not be used.
      •To minimize denaturation of serum lipoproteins and turbidity, it is recommended to prepare single-use aliquots and avoid repeated freeze-thaw cycles.

      Helpful?

  4. Is this product F7524 heat-inactivated? Should it be prior to use?

    1 answer

    1. This product has not been heat inactivated. For most cell culture applications heat inactivation is not necessary. If the application requires inactivation of any heat labile serum components, such as compliment or cytokines, the product may be heat inactivated in the lab. Alternatively, a heat inactivated version of this product is also available. See product F9665.

      To heat inactivate this product:
      1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.
      2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.
      3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.
      4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Avoid repeated thaw-freeze cycles.

      Helpful?

  5. Bonjour, Quelle est la composition et la concentration du sérum référencé F7524 en Calcium ? Cordialement

    1 answer

    1. Calcium levels are not determined for this product. The serum is a natural product that will contain calcium at physiological levels.

      Helpful?

  6. Welches fetale Kälbserum wurde für die Kryokonservierung von Mausembryonen oder Eiestockmäus verwendet?

    1 answer

    1. Unfortunately, a fetal calf serum that has been specifically tested for cryopreservation of mouse embryos or ovarian mice is not currently offered. Please see the link below to review Product ES009-M, Fetal Bovine Serum. This product is tested for expansion and cytotoxicity of mouse embryonic stem cells and may be of interest:
      https://www.sigmaaldrich.com/product/mm/es009m

      Helpful?

  7. How can we heat inactivate the fetal bovine serum cat: F7524? For how long?

    1 answer

    1. All heat inactivated serum products are treated at 56 deg. C for 30 minutes. This process may be applied to manually heat inactivate serum on a per bottle basis using a water bath. When immersing bottles do not allow water to come in contact with the cap/closure.

      Helpful?

  8. Is Feta bovine serum F7524-500ML heat inactivated?

    1 answer

    1. This product is not heat-inactivated.

      Helpful?

  9. Is Product F7524, Fetal Bovine Serum, available in the US or Canada?

    1 answer

    1. No, Product No. F7524 is only available in Europe.

      Helpful?

  10. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

1–10 of 16 Questions  

Reviews

1 of 8 reviewers received a sample product or took part in a promotion
1–2 of 8 Reviews  

Active Filters

  1. TU Kaiserslautern
    • Review 1
    • Votes 5
    5 out of 5 stars.

    Fetal Bovine Serum

    Serum tested and found to be suitable for HeLa, RPE and C2C12 cells.

    Helpful?

  2. Poznan, Poland
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Works correctly with C2C12 cells within 10-20% concentration in medium

    Helpful?

  3. 6 Ratings-Only Reviews

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