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C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Szinonimák:

blocking buffer

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
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About This Item

EC-szám:
232-555-1
UNSPSC kód:
12161700
NACRES:
NA.25

grade

for molecular biology

Minőségi szint

200

form

powder blend

technika/technikák

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

oldhatóság

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

tárolási hőmérséklet

room temp

Related Categories

Általános leírás

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Alkalmazás

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Tulajdonságok és előnyök

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Elkészítési megjegyzés

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

kapcsolódó termék

Product No.
Leírás
Árazás

P7059

Phosphate Buffered Saline, 10× PBS for Western blots and IP

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

H7140

Hybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

H7033

PerfectHyb™ Plus Hybridization Buffer, for Northern and Southern blotting, solution

D9905

Denhardt′s Solution, for Northern and Southern blotting, lyophilized powder

D2532

Denhardt′s Solution 50x, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

85639

SSC Buffer, for Northern and Southern blotting, powder blend

93017

SSC Buffer 20x, for Northern and Southern blotting, solution

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Protocols

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern & Southern Blot protokollok

Ismerje meg a Northern és Southern blotting alapjait, a makromolekulák membrántartókra történő átvitelének protokolljaival és alkalmazásaival.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással