Összes fotó(1)
Fontos dokumentumok
C4478
S-Gal®/LB Agar Blend
reagent for selection of recombinant bacterial clones
Szinonimák:
Agar Blend
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41106200
NACRES:
NA.85
Javasolt termékek
grade
for molecular biology
Minőségi szint
sterilitás
non-sterile
Forma
powder
technika/technikák
microbiological culture: suitable
alkalmasság
suitable for β-galactosidase test
nonselective for Escherichia coli
nonselective for coliforms
alkalmazás(ok)
food and beverages
microbiology
tárolási hőmérséklet
room temp
Related Categories
Általános leírás
S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Alkalmazás
S-Gal®/LB Agar Blend has been used for the identification of recombinant bacterial colonies.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® (sodium salt) is water-soluble, autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
Tulajdonságok és előnyök
- More intense color contrast than X-gal
- Water-soluble and autoclavable for easiest use
- Convenient, pre-mixed media
Komponensek
Ingredients (g/L)
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Tryptone, 10
Yeast extract, 5
Sodium chloride, 10
Agar, 12
S-Gal, 0.3
Ferric ammonium citrate, 0.5
IPTG, 0.03
Elv
When S-Gal® is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.
Elkészítési megjegyzés
Suspend contents of one packet in 500 ml distilled or deionized water. Sterilize by autoclaving for 15 to 20 minutes at 121-124°C. For microwaving, heat suspended mix until initial boiling. Mix well. Heat for short intervals with mixing until agar component is in solution. Do not allow boiling for extended periods of time. Antibiotics should be added following autoclaving or microwaving, after cooling to 48-52°C.
Egyéb megjegyzések
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.
Jogi információk
S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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