Ugrás a tartalomra
Merck

S9811

Sigma-Aldrich

S-Gal®

reagent for selection of recombinant bacterial clones

Szinonimák:

3,4-Cyclohexenoesculetin β-D-galactopyranoside

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

Tapasztalati képlet (Hill-képlet):
C19H22O9
CAS-szám:
Molekulatömeg:
394.37
UNSPSC kód:
12352200
PubChem Substance ID:
NACRES:
NA.85

grade

for molecular biology

Minőségi szint

sterilitás

non-sterile

Teszt

≥95% (HPLC)

Forma

powder

oldhatóság

DMF: 50 mg/mL

alkalmasság

suitable for β-galactosidase test

tárolási hőmérséklet

room temp

SMILES string

OC[C@H]1O[C@@H](Oc2cc3OC(=O)C4=C(CCCC4)c3cc2O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1

Nemzetközi kémiai azonosító kulcs

HDJJDRXZHJRVGA-DIKXUDHVSA-N

Általános leírás

S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Alkalmazás

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.
S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Tulajdonságok és előnyök

  • More intense color contrast than X-gal
  • Excellent for use in automated colony counters
  • Autoclavable for easiest use
  • No need to make stock solutions
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.

Egyéb megjegyzések

Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

Elv

When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.

Feloldás

Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.

Jogi információk

S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany

Piktogramok

Exclamation mark

Figyelmeztetés

Warning

Figyelmeztető mondatok

Veszélyességi osztályok

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Célzott szervek

Respiratory system

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

dust mask type N95 (US), Eyeshields, Gloves


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Cikkek

Általános protokollok a kompetens sejtek növekedésére és transzformációjára (DNS felvételére).

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Technikai cikk a kompetens sejtekről. A transzformáció olyan folyamat, amelynek során egyes baktériumok idegen genetikai anyagot (csupasz DNS-t) vesznek fel a környezetből.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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