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Merck

C40

Sigma-Aldrich

Catalase from bovine liver

lyophilized powder, ≥10,000 units/mg protein

Szinonimák:

H2O2:H2O2 oxidoreductase

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

100 MG
36 600,00 Ft
500 MG
106 000,00 Ft
1 G
146 000,00 Ft

36 600,00 Ft


Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.

Megrendelés igénylése nagy tételben

Méret kiválasztása

Nézet módosítása
100 MG
36 600,00 Ft
500 MG
106 000,00 Ft
1 G
146 000,00 Ft

About This Item

CAS-szám:
Enzyme Commission szám:
EC-szám:
MDL-szám:
UNSPSC kód:
12352204
eCl@ss:
32160410
NACRES:
NA.54

36 600,00 Ft


Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.

Megrendelés igénylése nagy tételben

Forma

lyophilized powder

Minőségi szint

specifikus aktivitás

≥10,000 units/mg protein

molekulatömeg

tetramer ~250 kDa

tartalmaz

≤0.2 wt. % Thymol

izoelektromos pont

5.4

oldhatóság

50 mM potassium phosphate buffer, pH 7.0: soluble 2.0 mg/mL

UniProt elérési szám

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

SMILES string

O(CC)C(=O)c1ccc(cc1)O

InChI

1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3

Nemzetközi kémiai azonosító kulcs

NUVBSKCKDOMJSU-UHFFFAOYSA-N

Géninformáció

cow ... CAT(280743)

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Általános leírás

Research Area: Cell Signaling

Catalase from bovine liver is a tetramer consisting of 4 equal subunits each with a 60 kDa molecular weight. Each of these subunits contains iron bound to a protoheme IX group. The enzyme will also strongly bind to NADP, where NADP and the heme group are within 13.7 angstroms.

Alkalmazás

Catalase acts as a natural antioxidant to study the roles of reactive oxygen species in gene expression and apoptosis. It has also been used to protect against oxidative damage to proteins, lipids, and nucleic acids. Industrially, catalzes have been used to remove hydrogen peroxide added to milk and cheese, in textile bleaching, and to examine its positive effects on the viability of DNA-repair mutants of E. coli.

Catalase from bovine liver may be used:

  • to prepare H2O2-O2 based biocathode for applications in glucose biofuel cells
  • to study the kinetic properties and storage stability of catalase immobilized on to florisil
  • in glutathione-mediated superoxide generation in an aqueous solution

Biokémiai/fiziológiai hatások

Catalase activity is constant over the pH range of 4.0-8.5. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu2+ results in degradation of the catalase molecule.
Catalase, an antioxidant enzyme found in all aerobic organisms, catalyzes the degradation of hydrogen peroxide, a byproduct of metabolic processes, into less harmful water and oxygen. It can also react with alkylhydrogen peroxides, such as methylperoxide and ethylperoxide and the second H2O2 molecule can be replaced by methanol, ethanol, propanol, formate and nitrate as a hydrogen donor. Catalase enzyme uses either iron (Fe) or manganese (Mn) as cofactor, and are classified as Fe-CAT or Mn-CAT.

Vigyázat

Solutions of catalse should not be frozen. Frozen solution will result in a 50-70% loss of activity.

Egység definíció

One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 concentration falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.

Egyéb megjegyzések

The protein content is determined by the Biuret method. In the product specifications, a protein content >= 70% is guaranteed. The impurities are not analyzed. The only other content specification is the concentration of Thymol, <= 0.2% wt.

Gátló

Product No.
Leírás
Árazás

Piktogramok

Health hazard

Figyelmeztetés

Danger

Figyelmeztető mondatok

Óvintézkedésre vonatkozó mondatok

Veszélyességi osztályok

Resp. Sens. 1

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number

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Ha egy adott verzióra van szüksége, a tétel- vagy cikkszám alapján rákereshet egy adott tanúsítványra.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Yifan Jiang et al.
Molecules (Basel, Switzerland), 25(7) (2020-04-12)
Clematis florida is a popular ornamental vine species known for diverse colors and shapes of its flowers but not for scent. Here we investigated the composition and biosynthesis of floral scent in 'Kaiser', a fragrant cultivar of C. florida that
Emilia M Sogin et al.
mSystems, 4(6) (2019-12-12)
Microbial communities exchange molecules with their environment, which plays a major role in regulating global biogeochemical cycles and climate. While extracellular metabolites are commonly measured in terrestrial and limnic ecosystems, the presence of salt in marine habitats limits the nontargeted
Afra Khiralla et al.
Microorganisms, 8(9) (2020-09-10)
An endophytic fungus isolated from Vernonia amygdalina, a medicinal plant from Sudan, was taxonomically characterized as Curvularia papendorfii. Ethyl acetate crude extract of C. papendorfii revealed an important antiviral effect against two viral pathogens, the human coronavirus HCoV 229E and
Florian Stehr et al.
Nature communications, 12(1), 4432-4432 (2021-07-23)
Monitoring biomolecules in single-particle tracking experiments is typically achieved by employing fixed organic dyes or fluorescent fusion proteins linked to a target of interest. However, photobleaching typically limits observation times to merely a few seconds, restricting downstream statistical analysis and
Fujun Luo et al.
Nature communications, 12(1), 2380-2380 (2021-04-24)
Diverse signaling complexes are precisely assembled at the presynaptic active zone for dynamic modulation of synaptic transmission and synaptic plasticity. Presynaptic GABAB-receptors nucleate critical signaling complexes regulating neurotransmitter release at most synapses. However, the molecular mechanisms underlying assembly of GABAB-receptor

Protocols

This procedure may be used for all Catalase products.

Questions

  1. Nelle specifiche leggo che il contenuto proteico di questa catalasi liofilizzata è del 70% Il restante 30% da cosa è composto ?

    1 answer
    1. The protein content is determined by the Biuret method. In the product specifications, a protein content >= 70% is guaranteed. As an example, lot number SLCL9662 reports a 90% protein content on the Certificate of Analysis (COA). The impurities are not analyzed. The only other content specification is the concentration of Thymol, <= 0.2% wt.

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