C3710
Ciliary Neurotrophic Factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Szinonimák:
CNTF
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
product name
Ciliary Neurotrophic Factor human, CNTF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biológiai forrás
human
Minőségi szint
rekombináns
expressed in E. coli
Teszt
≥95% (SDS-PAGE)
form
lyophilized powder
hatékonyság
≤325 ng/mL ED50 ((≥ 3.1 x 103 units/mg))
minőség
endotoxin tested
molekulatömeg
22.8 kDa
kiszerelés
pkg of 10 and 20 μg
tárolási körülmény
avoid repeated freeze/thaw cycles
technika/technikák
cell culture | mammalian: suitable
szennyeződések
≤10 EU/μg
UniProt elérési szám
tárolási hőmérséklet
−20°C
Géninformáció
human ... CNTF(1270) , CNTF (1270)
Általános leírás
The CNTF (ciliary neurotrophic factor) gene encodes a cytokine, and is mapped to human chromosome 11q12.1.
Alkalmazás
Ciliary Neurotrophic Factor human has been used to study its effect on photoreceptor neuroprotection and Muller glial cell proliferation in zebrafish retina. It has also been used to study the effects of intravitreal injection of ciliary neurotrophic factor (CNTF) on photoreceptor injury and BRB (blood-retinal barrier) breakdown in transgenic model.
Biokémiai/fiziológiai hatások
Ciliary neurotrophic factor (CNTF) was first identified as a survival factor for neurons from the ciliary ganglion of chicken embryos. Most of its known actions are restricted to cells of the nervous system, including motor neurons, sympathetic ganglion neurons, sensory neurons, hippocampal neurons, and medial septal neurons. CNTF also prevents degeneration of motor axons after axotomy and promotes astrocyte differentiation and oligodendrocyte survival and maturation. Outside the nervous system, CNTF maintains embryonic stem cells in an undifferentiated, pluripotent state. CNTF is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). CNTF exerts its actions through the activation of the high-affinity CTNF receptor complex, which contains the ligand-binding α-subunit (CNTF Rα) and two signal transducing β-subunits (LIF Rβ and gp130). The LIF Rβ subunit is also shared by receptors for LIF and OSM. The gp130 subunit is also shared by receptors for LIF, OSM, IL-6, and IL-11. CNTF is localized in the cell nucleus subsequent to receptor binding. Human and rat CNTF share ~83% sequence homology and show cross-reactivity in bioactivity.
Fizikai forma
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Analízis megjegyzés
The proliferative activity is tested in a cell proliferation assay using the cytokine-dependent human erythroleukemic cell line, TF-1.
Tárolási osztály kódja
13 - Non Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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CNTF induces photoreceptor neuroprotection and Muller glial cell proliferation through two different signaling pathways in the adult zebrafish retina
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