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SRP3014

Sigma-Aldrich

BDNF human

Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture

Szinonimák:

Brain Derived Neurotrophic Factor

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez

Méret kiválasztása

10 μG
171 000,00 Ft

171 000,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben
Összes fotó(3)

Méret kiválasztása

Nézet módosítása
10 μG
171 000,00 Ft

About This Item

UNSPSC kód:
12352202
NACRES:
NA.32

171 000,00 Ft

Az elérhetőséggel kapcsolatos kérdésekkel kérjük, forduljon Vevőszolgálatunkhoz.
Megrendelés igénylése nagy tételben

biológiai forrás

human

Minőségi szint

200

rekombináns

expressed in E. coli

Teszt

≥95% (SDS-PAGE)

Forma

lyophilized

molekulatömeg

27.0 kDa

kiszerelés

pkg of 10 μg

technika/technikák

cell culture | mammalian: suitable

szennyeződések

≤1.00 EU/μg endotoxin, tested

UniProt elérési szám

P23560

kiszállítva

wet ice

tárolási hőmérséklet

−20°C

Géninformáció

human ... BDNF(627)

Általános leírás

The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1.[1] BDNF is a member of the neurotrophin family of growth factors.[2][3] The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling.[4] BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions.

Biokémiai/fiziológiai hatások

Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B).[2] BDNF also plays an important role in vascular function and participates in angiogenesis.[5] It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.[4]

Szekvencia

MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Fizikai forma

Lyophilized with no additives.

Feloldás

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Analízis megjegyzés

The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000348742
0000282587
0000251766
0000251765
0000282587

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COA keresése

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Dokumentumtár megtekintése

Nia M Harris et al.
Pharmacology, biochemistry, and behavior, 150-151, 48-56 (2016-10-26)
Low levels of brain-derived neurotrophic factor (BDNF) are linked to delayed neurological recovery, depression, and cognitive impairment following stroke. Supplementation with BDNF reverses these effects. Unfortunately, systemically administered BDNF in its native form has minimal therapeutic value due to its
Elisabetta Palazzo et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Well-regulated epidermal homeostasis depends on the function of different classes of factors, such as transcription regulators and receptors. Alterations in this homeostatic balance may lead to the development of cutaneous squamous tumorigenesis. The homeobox transcription factor DLX3 is determinant for
Function and evolution in the NGF family and its receptors.
Journal of Neuroscience Research, 32, 461-470 (1992)
Cherian K Kandathil et al.
Hearing research, 342, 134-143 (2016-10-25)
Many previous studies have shown significant neurotrophic effects of intracochlear delivery of BDNF in preventing degeneration of cochlear spiral ganglion (SG) neurons after deafness in rodents and our laboratory has shown similar results in developing cats deafened prior to hearing
Ruhui Lin et al.
Molecular medicine reports, 13(2), 1611-1617 (2016-01-08)
Alzheimer's disease (AD) is a common human neurodegenerative disorder characterized by progressive deterioration of cognition and memory. Acupuncture at the Baihui (DU20) acupoint has long been used in China to clinically treat cognitive impairment. However, the precise mechanism underlying its
View All Related Papers

Questions

  1. We ordered rBDNF (SRP3014-10UG) to be used for cell culture . As we plan to store the compound for more than a week. What other stabilizer compound asiide  from 5% Trehalose can we use to store the compound longer once reconstituted. Thank you.

    1 answer

    1. This product is carrier free and has not been tested for long term solution stability. The addition of a carrier, such as BSA or HAS at 0.1%, would be expected to improve the long term stability of this material in solution. Product B3795 is a BDNF product that includes BSA as a carrier protein. This item may be stored in solution for up to 1 week at 2-8 °C or stored in working aliquots at –20 °C for several months.

      Please see the link below to review this product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/185/661/b3795dat-ms.pdf

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