SRP3014
BDNF human
Carrier free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), suitable for cell culture
Szinonimák:
Brain Derived Neurotrophic Factor
Bejelentkezés a szervezeti és szerződéses árak megtekintéséhez.
Méret kiválasztása
Erről a tételről
UNSPSC kód:
12352202
NACRES:
NA.32
Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?Műszaki ügyfélszolgálat
Segítségre van szüksége? Szakértő tudósaink csapata készséggel áll az Ön rendelkezésére.
Segíthetünk?biológiai forrás
human
Minőségi szint
rekombináns
expressed in E. coli
Teszt
≥95% (SDS-PAGE)
Forma
lyophilized
molekulatömeg
27.0 kDa
kiszerelés
pkg of 10 μg
technika/technikák
cell culture | mammalian: suitable
szennyeződések
≤1.00 EU/μg endotoxin, tested
UniProt elérési szám
kiszállítva
wet ice
tárolási hőmérséklet
−20°C
Géninformáció
human ... BDNF(627)
Általános leírás
The gene BDNF (brain derived neurotrophic factor) is mapped to human chromosome 11p14.1. BDNF is a member of the neurotrophin family of growth factors. The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0kDa homodimer of two 119 amino acid subunits linked by strong noncovalent interactions.
Biokémiai/fiziológiai hatások
Like other members of this family, BDNF (brain derived neurotrophic factor) supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR (low-affinity nerve growth factor receptor), which also binds other neurotrophins such as NGF (nerve growth factor), NT-3 (neurotrophin-3) and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB (tropomyosin-related kinase B). BDNF also plays an important role in vascular function and participates in angiogenesis. It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.
Fizikai forma
Lyophilized with no additives.
Elkészítési megjegyzés
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Analízis megjegyzés
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Egyéb megjegyzések
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Function and evolution in the NGF family and its receptors.
Journal of Neuroscience Research, 32, 461-470 (1992)
Elisabetta Palazzo et al.
International journal of molecular sciences, 20(14) (2019-07-25)
Well-regulated epidermal homeostasis depends on the function of different classes of factors, such as transcription regulators and receptors. Alterations in this homeostatic balance may lead to the development of cutaneous squamous tumorigenesis. The homeobox transcription factor DLX3 is determinant for
Nia M Harris et al.
Pharmacology, biochemistry, and behavior, 150-151, 48-56 (2016-10-26)
Low levels of brain-derived neurotrophic factor (BDNF) are linked to delayed neurological recovery, depression, and cognitive impairment following stroke. Supplementation with BDNF reverses these effects. Unfortunately, systemically administered BDNF in its native form has minimal therapeutic value due to its
Cherian K Kandathil et al.
Hearing research, 342, 134-143 (2016-10-25)
Many previous studies have shown significant neurotrophic effects of intracochlear delivery of BDNF in preventing degeneration of cochlear spiral ganglion (SG) neurons after deafness in rodents and our laboratory has shown similar results in developing cats deafened prior to hearing
Hai Yin Hu et al.
Cell reports, 27(3), 666-675 (2019-04-18)
Dendritic inhibitory synapses are most efficient in modulating excitatory inputs localized on the same dendrite, but it is unknown whether their location is random or regulated. Here, we show that the formation of inhibitory synapses can be directed by excitatory
Protokollok
Discover an expert protocol for seeding and culturing midbrain dopaminergic neurons in monoculture. Explore high purity cells and culture reagents.
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