Összes fotó(3)
Fontos dokumentumok
B3795
Brain-derived neurotrophic factor human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Szinonimák:
Abrineurin, BDNF
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(3)
About This Item
Javasolt termékek
Terméknév
Brain-derived neurotrophic factor human, BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biológiai forrás
human
Minőségi szint
rekombináns
expressed in E. coli
Teszt
≥95% (SDS-PAGE)
Forma
lyophilized powder
minőség
endotoxin tested
molekulatömeg
~27 kDa
kiszerelés
pkg of 10 μg
pkg of 5 μg
tárolási körülmény
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technika/technikák
cell culture | mammalian: suitable
szennyeződések
≤1.00 EU/μg Endotoxin level
UniProt elérési szám
tárolási hőmérséklet
−20°C
Általános leírás
BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Alkalmazás
Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.
Biokémiai/fiziológiai hatások
Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Fizikai forma
lyophilized from a sterile 0.2 μm filtered solution with BSA
Analízis megjegyzés
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Analitikai tanúsítványok (COA)
Lot/Batch Number
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Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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