A8481

Anti-γ-Actin antibody, Mouse monoclonal

clone 2-2.1.14.17, purified from hybridoma cell culture

• Szinonimák: Anti-ACTG1
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ELISA (i); IHC; WB
• klón: 2-2.1.14.17, monoclonal
• faj reaktivitás: mouse, chicken, canine, bovine, human, hamster
• citations: 36
• technika/technikák: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: purified from hybridoma cell culture
• antitest terméktípus: primary antibodies
• klón: 2-2.1.14.17, monoclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen ~43 kDa
• faj reaktivitás: mouse, chicken, canine, bovine, human, hamster
• kiszerelés: antibody small pack of 25 μL
• koncentráció: ~2 mg/mL
• technika/technikák: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.25-0.5 μg/mL using total cell extract of 3T3 cells
• izotípus: IgG1
• UniProt elérési szám: P63261
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: bovine ... ACTG1(404122) ; chicken ... ACTG1(415296) ; human ... ACTG1(71) ; mouse ... Actg1(11465)

Leírás

Általános leírás

γ-Actin is mainly present in sarcolemma and to a lesser extent in reticular lattice of normal muscles. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Monoclonal Anti-γ-Actin (mouse IgG1 isotype) is derived from the hybridoma 2-2.1.14.17 produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice. Actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms. Actin isoforms show more than 90% overall sequence homology, but only 50-60% homology in their 18 NH₂-terminal residues. Actin constitutes for around 15% of the total protein. It exists in globular and filamentous forms.

Egyediség

Monoclonal Anti-γ-Actin recognizes human, bovine, canine, mouse, hamster and chicken γ-actin.

Alkalmazás

Anti-γ-Actin antibody, Mouse monoclonal has been used in :

• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• western blotting
• immunohistochemistry

Mouse monoclonal Anti-γ-Actin antibody has been used for immunofluorescence assays. The antibody can also be used for ELISA, IHC and western blot assays.

Protein lysates from human Jurkat T-leukemic cells were subjected to western blot and probed using monoclonal mouse anti-actin (AC-40) to ensure equal loading.

Biokémiai/fiziológiai hatások

The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin is significantly involved in the microfilament system. It participates in muscle contraction and gene regulation. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular process including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Increased levels of γ-actin have been linked to dystrophin loss and subsequent cell signaling and gene expression defects.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Egyéb megjegyzések

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)