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Merck

65906

Sigma-Aldrich

Phalloidin–Atto 647N

BioReagent, suitable for fluorescence, ≥80% (HPLC)

Szinonimák:

Atto 647N, Atto 647N-Phalloidin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12171501
NACRES:
NA.32

termékcsalád

BioReagent

Minőségi szint

Teszt

≥80% (HPLC)

gyártó/kereskedő neve

ATTO-TEC GmbH

λ

in methanol

UV-elnyelés

λ: 640-646 nm Amax

alkalmasság

suitable for fluorescence

észlelési módszer

fluorometric

tárolási hőmérséklet

−20°C

Általános leírás

Phalloidin–Atto 647N is a new fluorescentlabel targeting the red spectral region. Atto 647N is a cationic dye, and postcoupling, it carries a net electrical charge of +1.Like most Atto labels, the absorption andfluorescence of Atto 647N are independent of pH between 2-11. Atto 647N issupplied in the form of a mixture containing two isomers having identicalfluorescence and absorption properties. Atto labels have rigid structures thatdo not show any cis-trans-isomerization.

Alkalmazás

Phalloidin–Atto 647Nis designed to be used for labelling DNA, RNA, or proteins. Fluorescentconjugates of phalloidin are used to label actin filaments for histologicalapplications. Some structural features of phalloidin are required for thebinding to actin.

Tulajdonságok és előnyök

Characteristic features of the Phalloidin Atto488 are:
  1. StrongAbsorption.
  2. HighFluorescence quantum yield.
  3. HighPhotostability.
  4. MinimalTriplet formation.
  5. GoodSolubility.
  6. Excellent Ozone Resistance.

Jogi információk

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Piktogramok

Skull and crossbones

Figyelmeztetés

Danger

Figyelmeztető mondatok

Veszélyességi osztályok

Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral

Tárolási osztály kódja

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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Analitikai tanúsítványok (COA)

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Dokumentumtár megtekintése

Georgios Trichas et al.
BMC biology, 6, 40-40 (2008-09-17)
Transgenic animals are widely used in biomedical research and biotechnology. Multicistronic constructs, in which several proteins are encoded by a single messenger RNA, are commonly used in genetically engineered animals. This is currently done by using an internal ribosomal entry
Catherine Pfefferli et al.
Nature communications, 8, 15151-15151 (2017-05-04)
The existence of common mechanisms regulating organ regeneration is an intriguing concept. Here we report on a regulatory element that is transiently activated during heart and fin regeneration in zebrafish. This element contains a ctgfa upstream sequence, called careg, which
Benedetta Artegiani et al.
Nature cell biology, 22(3), 321-331 (2020-03-04)
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. Here, we describe CRISPR-Cas9-mediated homology-independent organoid transgenesis (CRISPR-HOT), which enables efficient generation
Francesca Farina et al.
The EMBO journal, 38(11) (2019-04-25)
Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule
Ceniz Zihni et al.
Nature cell biology, 19(9), 1049-1060 (2017-08-22)
Polarized epithelia develop distinct cell surface domains, with the apical membrane acquiring characteristic morphological features such as microvilli. Cell polarization is driven by polarity determinants including the evolutionarily conserved partitioning-defective (PAR) proteins that are separated into distinct cortical domains. PAR

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