Összes fotó(1)
Fontos dokumentumok
49429
Phalloidin–Atto 532
suitable for fluorescence, ≥90.0% (HPLC)
Szinonimák:
Atto 532–Phalloidin
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352108
NACRES:
NA.32
Javasolt termékek
Minőségi szint
Teszt
≥90.0% (HPLC)
gyártó/kereskedő neve
ATTO-TEC GmbH
λ
in acetonitrile: water (1:9) (with 0.01% trifluoroacetic acid)
UV-elnyelés
λ: 534-540 nm Amax
alkalmasság
suitable for fluorescence
tárolási hőmérséklet
−20°C
Általános leírás
Atto 532 is a novel fluorescent label related to the well-known dye Rhodamine 6G. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, excellent water solubility, and very little triplet formation. After coupling to a substrate Atto 532 Phalloidin carries a net electrical charge of -1. Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10–8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours.
Alkalmazás
Fluorescent conjugates of phalloidin, rhodamine-phalloidin staining reagents, such as Phalloidin–Atto 532 are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
Jogi információk
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Cikkek
Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.
Az Atto színezékek olyan fluoreszcens színezékek sorozata, amelyek megfelelnek a modern fluoreszcens technológiák kritikus igényeinek.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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