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Merck

18373

Sigma-Aldrich

Atto 647N NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

MDL-szám:
UNSPSC kód:
12352108
NACRES:
NA.32

termékcsalád

BioReagent

Minőségi szint

Teszt

≥90% (HPLC)
≥90% (degree of coupling)

Forma

solid

gyártó/kereskedő neve

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV-elnyelés

λ: 641-647 nm Amax

alkalmasság

suitable for fluorescence

tárolási hőmérséklet

−20°C

Általános leírás

Atto 647N is a superior red-emitting fluorescence dye with a strong absorption, excellent fluorescence quantum yield (65%), high photostability, excellent ozone resistance, good solubility, and very little triplet formation.

Alkalmazás

Atto 647N belongs to a new generation of fluorescent labels for the red spectral region. The dye is designed for application in the area of life science, e.g. labeling of DNA, RNA or proteins. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, high photostability, excellent ozone resistance, good solubility, and very little triplet formation. Atto 647N is a cationic dye. The active ester of Atto 647N fluorescence dye reacts with amino groups under mild conditions. After coupling to a substrate the dye carries a net electrical charge of +1. In common with most Atto-labels, absorption and fluorescence are independent of pH in the range of 2 to 11, used in typical applications. As supplied Atto 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties.

Jogi információk

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Ioannis Sgouralis et al.
The journal of physical chemistry. B, 123(3), 675-688 (2018-12-21)
We develop a Bayesian nonparametric framework to analyze single molecule FRET (smFRET) data. This framework, a variation on infinite hidden Markov models, goes beyond traditional hidden Markov analysis, which already treats photon shot noise, in three critical ways: (1) it
Jialei Tang et al.
Scientific reports, 7(1), 10945-10945 (2017-09-10)
We report a simple single-molecule fluorescence imaging method that increases the temporal resolution of any type of array detector by >5-fold with full field-of-view. We spread single-molecule spots to adjacent pixels by rotating a mirror in the detection path during
Shangguo Hou et al.
Nature communications, 11(1), 3607-3607 (2020-07-19)
To date, single molecule studies have been reliant on tethering or confinement to achieve long duration and high temporal resolution measurements. Here, we present a 3D single-molecule active real-time tracking method (3D-SMART) which is capable of locking on to single
Jennifer Z Yao et al.
Journal of the American Chemical Society, 134(8), 3720-3728 (2012-01-14)
Methods for targeting of small molecules to cellular proteins can allow imaging with fluorophores that are smaller, brighter, and more photostable than fluorescent proteins. Previously, we reported targeting of the blue fluorophore coumarin to cellular proteins fused to a 13-amino
Feng Ye et al.
The Journal of endocrinology, 206(1), 27-36 (2010-04-16)
Adipose tissue is a highly active endocrine organ secreting a variety of signaling molecules called adipokines. Leptin and resistin are two adipokines critically involved in metabolic homeostasis. Nevertheless, the secretory pathways of these adipokines and their interplays are poorly elucidated.

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