60615

Kanamycin sulfate

mixture of Kanamycin A (main component) and Kanamycin B and C

• Lineáris képlet: C₁₈H_36-37N_4-5O_10-11 · H₂SO₄
• CAS-szám: 70560-51-9
• Beilstein: 3874279
• MDL-szám: MFCD00070253
• UNSPSC kód: 51281654
• PubChem Substance ID: 57651778
• NACRES: NA.85

Tulajdonságok

• Minőségi szint: 200
• form: powder or crystals
• hatékonyság: ≥750 units per mg (calculated with reference to dried substance)
• izzítási maradék: ≤0.5% (as SO₄)
• veszteség: ≤1.5% loss on drying
• szín: white to off-white
• antibiotikus hatásspektrum: Gram-negative bacteria; Gram-positive bacteria; mycobacteria; mycoplasma
• Hatásmechanizmus: protein synthesis | interferes
• SMILES string: OS(O)(=O)=O.NC[C@H]1O[C@@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• Nemzetközi kémiai azonosító kulcs: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Leírás

Általános leírás

Chemical structure: aminoglycoside

Alkalmazás

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium.†† Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 μg/mL.

Biokémiai/fiziológiai hatások

Mode of Action: The product acts by binding to the 70S ribosomal subunit, inhibiting translocation and eliciting miscoding.

Mode of Resistance: Aminoglycoside-modifying enzymes (including acetyltransferase, phosphotransferase, nucleotidyltransferase) can alter this antibiotic, preventing its interaction with ribosomes.

Antimicrobial Spectrum: Kanamycin sulfate is effective against gram-negative and gram-postiive bacteria, and mycoplasma.

Kiszerelés

5g, 25 g

Vigyázat

Solutions are stable at 37°C for approximately 5 days. Aqueous stock solutions can be stored at 2-8°C for long term storage.

Elkészítési megjegyzés

Kanamycin sulfate is soluble in water at 50 mg/mL, yielding a clear solution. It is practically insoluble in alcohol, acetone, chloroform, ether and ethyl acetate. A 1% solution in water has a pH of 6.5 to 8.5. Sterile solutions can be prepared by a sterile filtration, through a .2μm filter.

Egyéb megjegyzések

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.Storage class (TRGS 510): Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H360
• Óvintézkedésre vonatkozó mondatok: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Veszélyességi osztályok: Repr. 1B
• Tárolási osztály kódja: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)