69385
Protein Standard Mix 15 - 600 kDa
for size exclusion chromatography
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
85151701
NACRES:
NA.24
Javasolt termékek
Minőségi szint
form
solid
molekulatömeg
15-600 kDa
analit kémiai osztály(ok)
amino acids, peptides, proteins
technika/technikák
gel permeation chromatography (GPC): suitable
alkalmazás(ok)
clinical
food and beverages
pharmaceutical
format
neat
tárolási hőmérséklet
−20°C
Általános leírás
The protein standard mix is a calibration standard to test and monitor performance of size exclusion chromatography (SEC) columns. It is a lyophilized mixture of molecular weight markers ranging from 15 kDa to 600 kDa.
Components
Thyroglobulin bovine MW ~ 670 000 Da
γ-globulins from bovine blood MW ~ 150 000 Da
Ovalbumin MW~ 44 300 Da
Ribonuclease A type I-A MW ~ 13 700 Da
p-aminobenzoic acid (pABA) MW ~ 137 Da
Components
Thyroglobulin bovine MW ~ 670 000 Da
γ-globulins from bovine blood MW ~ 150 000 Da
Ovalbumin MW~ 44 300 Da
Ribonuclease A type I-A MW ~ 13 700 Da
p-aminobenzoic acid (pABA) MW ~ 137 Da
Alkalmazás
This analytical standard is used for the following:
- Evaluation of selectivity and separation efficiency of size exclusion chromatography (SEC) to separate intact proteins by varying flow rate, size of silica particles and pore sizes in the column
- Simultaneous determination of oligomerized and nitrated proteins by size exclusion chromatography-high performance liquid chromatography-diode array detection (SEC-HPLC-DAD)
- Molecular weight separation of proteins by size-exclusion chromatography, formed upon O3 and NO2 induced oxidation, nitration, and oligomerization of bovine serum albumin (BSA) as a model protein
- Estimation of molecular masses of two recombinant proteins— TNF fluorescent sensor (BTN-Kat) and fluorescent sensor-inhibitor (ITN-Kat), by size exclusion chromatography (SEC) to evaluate their ability of binding and neutralizing tumor necrosis factor (TNF) in vitro and further serving as imaging labels for non-invasive analysis
kapcsolódó termék
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Resp. Sens. 1
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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Az ügyfelek ezeket is megtekintették
Different Structural Conformers of Monomeric α-Synuclein Identified after Lyophilizing and Freezing.
Amberley D Stephens et al.
Analytical chemistry, 90(11), 6975-6983 (2018-05-12)
Understanding the mechanisms behind amyloid protein aggregation in diseases, such as Parkinson's and Alzheimer's disease, is often hampered by the reproducibility of in vitro assays. Yet, understanding the basic mechanisms of protein misfolding is essential for the development of novel
Christopher J Kampf et al.
Environmental science & technology, 49(18), 10859-10866 (2015-08-20)
Air pollution is a potential driver for the increasing prevalence of allergic disease, and post-translational modification by air pollutants can enhance the allergenic potential of proteins. Here, the kinetics and mechanism of protein oligomerization upon ozone (O3) exposure were studied
Thomas Fricke et al.
Viruses, 14(3) (2022-03-27)
Kaposi's sarcoma herpesvirus (KSHV) is associated with a significant disease burden, in particular in Sub-Sahara Africa. A KSHV vaccine would be highly desirable, but the mechanisms underlying neutralizing antibody responses against KSHV remain largely unexplored. The complex made of glycoproteins
Andrew J Bowling et al.
Toxins, 11(5) (2019-05-19)
Vip3A proteins are important for the control of spodopteran pests in crops, including Spodoptera frugiperda (fall armyworm). Native Vip3Ab1 controls S. frugiperda, but it is ineffective against S. eridania (southern armyworm), a major pest of soybean in South America. Recently
Robert C Monsen et al.
Nucleic acids research, 49(3), 1749-1768 (2021-01-21)
Human telomeres contain the repeat DNA sequence 5'-d(TTAGGG), with duplex regions that are several kilobases long terminating in a 3' single-stranded overhang. The structure of the single-stranded overhang is not known with certainty, with disparate models proposed in the literature.
Cikkek
Size-exclusion chromatography (SEC) columns and ready-to-use standards facilitate method development and increase robustness of protein SEC methods.
Characterize mAb monomers, aggregates, and fragments using SEC-UV workflow with Zenix® and Zenix®-C SEC columns.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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