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S5171

Sigma-Aldrich

Human Fibronectin

formulated with recombinant human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma, liquid, 2 mg/mL, suitable for cell culture

Szinonimák:

sFN

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00284706
UNSPSC kód:
12352202
NACRES:
NA.75

Terméknév

Superfibronectin from human plasma, solution, BioReagent, suitable for cell culture

biológiai forrás

human

Minőségi szint

200

rekombináns

expressed in E. coli

termékcsalád

BioReagent

Forma

solution

molekulatömeg

~235-270 kDa

kiszerelés

pkg of 5 mg

technika/technikák

cell culture | mammalian: suitable

felület bevonatolás

1 μg/cm2

UniProt elérési szám

P02751

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

Géninformáció

human ... FN1(2335)

Alkalmazás

Superfibronectin from human plasma dramatically enhances the adhesive properties of fibronectin and suppresses cell migration. Improved results have been shown CHO cells, IMR-90 fibroblasts, T47D breast carcinoma cells, and UCLA-P lung carcinoma cells. Additional applications include epithelial cells, mesenchymal cells, neuronal cells, fibroblasts, neural crest cells, and endothelial cells. It is recommended for use as a cell culture substratum at 1 μg/cm2.

Komponensek

Superfibronectin is formulated with recombinant, human fibronectin fragment III-C expressed in E. coli and fibronectin from human plasma. The recombinant fibronectin includes a new region, type III, cloned from human placenta cDNA, that is newly reported to participate in matrix formation by inducing spontaneous disulfide crosslinking of fibronectin molecules into multimers. The product resembles the in vivo matrix form of fibronectin.

Vigyázat

Superfibronectin should be stored at 2-8°C and diluted immediately prior to use. Do not freeze this product.

Elkészítési megjegyzés

As supplied, this product is a 2 mg protein/mL solution in 0.05 M Tris buffered saline. Before coating a 96 well plate, it is recommended to dilute t he product with sterile PBS to a concentration of 5 μg/mL and add 100 μl/well. These plates should then be incubated for 2 hours at 37°C and washed twice with 100 μl of PBS. The source material has been tested for HBsAg and HIV antibodies.

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 2

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

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Analitikai tanúsítványok (COA)

Lot/Batch Number
0000388233
0000388233
0000198891
11180741
0000117157

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Dokumentumtár megtekintése

Gentle cell handling with an ultra-slow instrument: creep-manipulation of cells
Thielecke H, et al.
Microscopica Acta, 11(11), 1230-1241 (2005)
N Okada et al.
The Journal of biological chemistry, 272(43), 26978-26984 (1997-10-27)
The pathogenic Gram-positive bacterium Streptococcus pyogenes (group A streptococcus) binds to fibronectin via protein F. In this study, we have investigated the binding properties of protein F to various multimeric tissue forms of fibronectin that appear on cell surfaces and
Regulated GPCR trafficking to the plasma membrane: general issues and the CCR5 chemokine receptor example
Giornale di Psichiatria e di Neuropatologia (2012)
A Morla et al.
Nature, 367(6459), 193-196 (1994-01-13)
Fibronectin is an extracellular matrix protein that is important in development, wound healing and tumorigenesis. In the blood it is dimeric, but in tissues forms disulphide crosslinked fibrils. Here we show that a fragment from the first type-III repeat of
Vladislav Belyy et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(3), 1533-1542 (2019-12-25)
The endoplasmic reticulum (ER) membrane-resident stress sensor inositol-requiring enzyme 1 (IRE1) governs the most evolutionarily conserved branch of the unfolded protein response. Upon sensing an accumulation of unfolded proteins in the ER lumen, IRE1 activates its cytoplasmic kinase and ribonuclease
View All Related Papers

Cikkek

Methods for Enhancing Lentiviral Transduction Efficiency

Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.

Fibronectin

Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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