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Merck

11796895001

Roche

DIG Easy Hyb Granules

greener alternative

powder, suitable for hybridization

Szinonimák:

DIG system

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41105326

Ügynökség

suitable for

Minőségi szint

Forma

powder

használat

sufficient for 6 x 100 mL

gyártó/kereskedő neve

Roche

tárolási körülmény

(Keep container tightly closed in a dry and well-ventilated place.)

környezetbarátabb alternatív termék tulajdonságai

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technika/technikák

hybridization: suitable

szín

white

pH

7,0

oldhatóság

water: soluble

alkalmasság

suitable for nucleic acid hybridization

környezetbarátabb alternatív kategória

tárolási hőmérséklet

20-25°C

Általános leírás

DIG Easy Hyb is a hybridization solution used for nucleic acid blots with digoxigenin-labeled probes. DIG Easy Hyb does not contain formamide but facilitates the lowering of hybridization temperature comparable to buffers that contain 50% formamide.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Alkalmazás

DIG Easy Hyb Granules can be used in all membrane hybridization applications using nonradioactive (e.g., digoxigenin-labeled) nucleic acid probes. It is specifically designed for use with DIG kits.

Tulajdonságok és előnyök

  • DIG Easy Hyb is a convenient ready-to-use hybridization buffer, saving time and effort.
  • DIG Easy Hyb aligns with the principles of Green Chemistry by offering a greener alternative for hybridization buffers.

Elkészítési megjegyzés

Working solution: Add carefully 64 ml sterile double-dist. water to the plastic bottle, dissolve by stirring immediately for 5 minutes at 37 °C. The availability of 6 x 100 ml portions allows you to freshly "produce" the DIG Easy Hyb when required—no long-term storage of a solution is required.
Note: For applications with Northern blots, DIG Easy Hyb Granules must be dissolved under RNase-free conditions.
Nontoxic granules, aliquoted in plastic bottles. Reconstitute to provide 100ml of a 1x solution, ready-to-use.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Jogi információk

DIG Easy Hyb is a trademark of Roche

Piktogramok

Exclamation mark

Figyelmeztetés

Warning

Figyelmeztető mondatok

Veszélyességi osztályok

Eye Irrit. 2 - Skin Irrit. 2

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 2

Lobbanási pont (F)

does not flash

Lobbanási pont (C)

does not flash


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Dokumentumtár megtekintése

Ru-Bing Duan et al.
The Journal of biological chemistry, 289(23), 16006-16015 (2014-04-24)
There are multiple isoforms of p90 ribosomal S6 kinase (RSK), which regulate diverse cellular functions such as cell growth, proliferation, maturation, and motility. However, the relationship between the structures and functions of RSK isoforms remains undetermined. Artemia is a useful
Yanchang Luo et al.
Plant biotechnology (Tokyo, Japan), 37(1), 69-75 (2020-05-05)
Retrotransposons are mobile genetic elements capable of transposition via reverse transcription of RNA intermediates. Rice cultivar Nipponbare contains two nearly identical genomic copies of Tos17, an endogenous copia-like LTR retrotransposon, on chromosomes 7 (Tos17 Chr.7) and 10 (Tos17 Chr.10), respectively.
Chih-Chiang Chan et al.
Current biology : CB, 21(20), 1704-1715 (2011-10-18)
Neurons require highly specialized intracellular membrane trafficking, especially at synapses. Rab GTPases are considered master regulators of membrane trafficking in all cells, and only very few Rabs have known neuron-specific functions. Here, we present the first systematic characterization of neuronal
An Li et al.
Fungal genetics and biology : FG & B, 48(6), 602-611 (2011-02-18)
Several Aspergillus species are well-known for the production of a variety of organic acids. In this study, a cloned based transcriptomics approach was used to identify genes crucial in the biosynthesis pathway for one of these acids, itaconic acid. From
Stefanie Wehner et al.
RNA biology, 11(11), 1467-1478 (2014-12-09)
6S RNA is a highly abundant small non-coding RNA widely spread among diverse bacterial groups. By competing with DNA promoters for binding to RNA polymerase (RNAP), the RNA regulates transcription on a global scale. RNAP produces small product RNAs derived

Protocols

DIG Easy Hyb™ Granules Protocol & Troubleshooting

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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