Összes fotó(1)
Fontos dokumentumok
11175041910
Roche
DIG Nucleic Acid Detection Kit
sufficient for 40 blots (10 cm x 10 cm each), kit of 1 (5 components), suitable for hybridization, suitable for Northern blotting
Szinonimák:
DIG system
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41116134
Javasolt termékek
használat
sufficient for 40 blots (10 cm x 10 cm each)
Minőségi szint
kiszerelés
kit of 1 (5 components)
gyártó/kereskedő neve
Roche
környezetbarátabb alternatív termék tulajdonságai
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technika/technikák
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
környezetbarátabb alternatív kategória
, Aligned
tárolási hőmérséklet
−20°C
Általános leírás
DIG Nucleic Acid Detection Kit is used for detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
DIG Nucleic Acid Detection Kit is used for the detection of digoxigenin-labeled nucleic acids by enzyme immunoassay and enzyme-catalyzed color reaction. DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP (alkaline phosphatase) antibody conjugate and the color substrates NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate).
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Alkalmazás
DIG nucleic acid detection kit has been used in the confirmation of genetic manipulation.
The DIG Nucleic Acid Detection Kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Other nucleic acid blotting applications
- In situ hybridization applications
The kit contains all essential reagents for the detection of membrane-blotted, digoxigenin-labeled nucleic acid hybrids. It can be used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Other nucleic acid blotting applications
- In situ hybridization applications
Tulajdonságok és előnyök
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Kiszerelés
1 kit containing 5 components.
Elv
DIG-labeled nucleic acids are detected after hybridization in an enzyme-linked immunoassay with a highly specific anti-DIG-AP antibody conjugate and the color substrates NBT and BCIP.
Elkészítési megjegyzés
Working concentration: Flow cytometry: 2 μg/ml (0.2 μg/100 μl/106 cells); Immunohistocytochemistry: 6 μg/ml
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
Working concentration of conjugate depends on application and substrate.
Storage conditions (working solution):
- Anti-Digoxigenin-AP Conjugate (vial 3): 2 to 8 °C, it is stable for 12 months at this temperature
- NBT/BCIP (vial 4): 2 to 8 °C, stable
Note: During shipment of the kit on dry ice, a precipitate may occur which is dissolved by briefly warming to 37 °C - The blocking reagent (vial 5) is stable for 36 months and can be stored dry at 4 to 8 °C.
- Autoclaved stock solution can be stored for several days to a week either unopened at 15 to 25 °C or at 4 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.
Egyéb megjegyzések
Kit Components Only
Product No.
Leírás
- DIG-labeled Control DNA 5 µg/ml
- DNA Dilution Buffer, [50 μg/ml] fish sperm DNA
- Anti-Digoxigenin-AP Conjugate antibody 750 U/ml
- NBT/BCIP, 50x stock solution 50x concentrated
- Blocking Reagent
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
does not flashNot applicable
Lobbanási pont (C)
does not flashNot applicable
Válasszon a legfrissebb verziók közül:
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Zheyong Xue et al.
Nature communications, 9(1), 604-604 (2018-02-11)
In flowering plants, the pollen coat protects the released male germ cells from desiccation and damage during pollination. However, we know little about the mechanism by which the chemical composition of the pollen coat prevents dehydration of pollen grains. Here
Hiromasa Funato et al.
Nature, 539(7629), 378-383 (2016-11-05)
Sleep is conserved from invertebrates to vertebrates, and is tightly regulated in a homeostatic manner. The molecular and cellular mechanisms that determine the amount of rapid eye movement sleep (REMS) and non-REMS (NREMS) remain unknown. Here we identify two dominant
Han Yang et al.
Nature communications, 13(1), 485-485 (2022-01-27)
Nitrogen (N), one of the most important plant nutrients, plays crucial roles in multiple plant developmental processes. Spikelets are the primary sink tissues during reproductive growth, and N deficiency can cause floral abortion. However, the roles of N nutrition in
Zhanwu Yang et al.
Plant biotechnology journal, 20(1), 103-115 (2021-09-07)
Legume-rhizobia symbiosis enables biological nitrogen fixation to improve crop production for sustainable agriculture. Small heat shock proteins (sHSPs) are involved in multiple environmental stresses and plant development processes. However, the role of sHSPs in nodule development in soybean remains largely
The role of Aspartyl aminopeptidase (Ape4) in Cryptococcus neoformans virulence and authophagy
Gontijo FA, et al.
PLoS ONE (2017)
Cikkek
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással