Összes fotó(1)
Fontos dokumentumok
11681451001
Roche
NBT/BCIP Stock Solution
Szinonimák:
5-bromo-4-chloro-3-indoyl phosphate, BCIP
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352204
Javasolt termékek
Forma
solution
Minőségi szint
molekulatömeg
817.7
kiszerelés
pkg of 8 mL
gyártó/kereskedő neve
Roche
tárolási hőmérséklet
2-8°C
Általános leírás
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Evaluation: visually
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Evaluation: visually
Alkalmazás
- The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
- Northern blot
- Western blot
- Colony and plaque lifts
- In situ hybridization
- and for applications in immunohistochemistry and immunocytochemistry.
Fizikai forma
Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.
Note: The color of the solution can vary from yellowish to light brown.
Elkészítési megjegyzés
Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
Always prepare this solution fresh shortly before use.
- For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
- For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 1
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
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