10901393001
Roche
Glycogen
from mussels
Szinonimák:
Glycogen, glycogen
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41116100
Javasolt termékek
Minőségi szint
form
solution
kiszerelés
pkg of 1 mL (20 mg)
gyártó/kereskedő neve
Roche
tárolási hőmérséklet
−20°C
Alkalmazás
This preparation is used as a carrier for the precipitation of nucleic acids (DNA or RNA). As an inert material it may replace tRNAs or sonicated DNAs.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.
20 μg glycogen (1 μl solution) allow to precipitate pg-amounts of DNA or RNA from a volume of 1 ml.
In a typical experiment 5 pg [3H]-labeled calf thymus DNA were dissolved in 500 μl 10 mM Tris-HCl, pH 8.0; 1 mM EDTA; 0.4 M LiCl. 1μl glycogen solution (20 μg glycogen) as carrier was added and then precipitated with 1.2 ml ethanol at -15 to -25 °C and stored for 3 hours at -15 to -25 °C. After centrifugation (10 minutes at 12 000 × g) the total radioactivity was found in the precipitate. Without addition of glycogen no precipitation of DNA occured.
Tulajdonságok és előnyök
Glycogen, in special quality for molecular biology, is an inert carrier in nucleic acid preparations.
Contents
Aqueous solution, 20 mg/ml
Contents
Aqueous solution, 20 mg/ml
Minőség
Tested for absence of endonucleases, nicking activity, exonucleases, RNases, nucleic acids, and proteases according to the current Quality Control procedures.
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
gyakran vásárolják ezzel a termékkel együtt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
nwg
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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